Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (1): 14-23.DOI: 10.13304/j.nykjdb.2021.0188
• AGRICULTURAL INNOVATION FORUM • Previous Articles Next Articles
Zhong ZHUANG1(), Long ZHAO1, Hao BAI2, Yulin BI1, Yingquan HUANG3, Guohong CHEN1, Guobin CHANG1(
)
Received:
2021-03-07
Accepted:
2021-06-21
Online:
2022-01-15
Published:
2022-01-25
Contact:
Guobin CHANG
庄重1(), 赵龙1, 白皓2, 毕瑜林1, 黄应权3, 陈国宏1, 常国斌1(
)
通讯作者:
常国斌
作者简介:
庄重 E-mail:1175007549@qq.com;
基金资助:
CLC Number:
Zhong ZHUANG, Long ZHAO, Hao BAI, Yulin BI, Yingquan HUANG, Guohong CHEN, Guobin CHANG. Development of CRISPR/Cas9 and Its Application in Poultry[J]. Journal of Agricultural Science and Technology, 2022, 24(1): 14-23.
庄重, 赵龙, 白皓, 毕瑜林, 黄应权, 陈国宏, 常国斌. CRISPR/Cas9技术在家禽育种方面的应用[J]. 中国农业科技导报, 2022, 24(1): 14-23.
家禽细胞模型 Poultry cell model | 靶基因 Target gene | 参考文献Reference |
---|---|---|
鸡淋巴瘤细胞(DT40)、鸡成纤维细胞(DF-1) Chicken lymphoma cell(DT40), chicken fibroblast(DF-1) | DROSHA,DICER,MBD3,KIAA1279,CDKN1B,EZH2,HIRA,TYRP1,STMN2,RET,DGCR | [ |
DF-1、鸡胚胎干细胞(ESCs) DF-1,chicken embryonic stem cells(ESCs) | C2EIP (2号染色体PGCs中表达) C2EIP (expression in PGCs on chromosome 2) | [ |
DF-1 | PPAR⁃γ (过氧化物酶体增殖物激活受体-γ)、ATP5E (ATP合成酶ε亚基)、OVA(卵清蛋白) PPAR⁃γ (peroxisome proliferator-activated receptor-γ),ATP5E (ATP synthase epsilon subunit),OVA(ovalbumin) | [ |
DF-1 | 肌肉生长抑制素Myostatin | [ |
鹌鹑肌肉细胞(QM7) Quail muscle cells(QM7) | MLPH (亲黑素位点) MLPH (melanophilin locus) | [ |
鸡原始生殖细胞(PGCs) Chicken primordial germ cells(PGCs) | CXCR4 (C-X-C趋化因子受体4型) CXCR4 (C-X-C chemokine receptor type 4) | [ |
ESCs | Stra8 (视黄酸激活基因8) Stra8 (stimulated by retinoic acid 8 gene) | [ |
DF-1 | GAPDH (甘油醛-3-磷酸脱氢酶) GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | [ |
DF-1 | TVA (抗A亚群禽白血病病毒受体)、TVC(抗C亚群禽白血病病毒受体)、TVJ(抗J亚群禽白血病病毒受体) TVA (anti A subgroup of avian leukemia virus receptor),TVC(anti C subgroup of avian leukemia virus receptor),TVJ(anti J subgroup of avian leukemia virus receptor) | [ |
DF-1 | TBK1 (TANK结合激酶1) TBK1 (TANK binding kinase 1) | [ |
DF-1 | DAZL (精症缺失样) DAZL (deleted in AZoospermia-like) | [ |
DF-1 | ANP32A (性核磷蛋白32A) ANP32A (acidic nuclear phosphoprotein 32 family,member A) | [ |
DF-1 | IRF7 (扰素调节因子7) IRF7(interferon regulator factor 7) | [ |
DF-1 | AMHR (缪勒管激素2型受体) AMHR (anti-mullerian hormone receptor type 2) | [ |
DF-1 | Apob (载脂蛋白B) Apob (apolipoprotein B) | [ |
Table 1 Application of CRISPR/Cas9 system in poultry basal cell model
家禽细胞模型 Poultry cell model | 靶基因 Target gene | 参考文献Reference |
---|---|---|
鸡淋巴瘤细胞(DT40)、鸡成纤维细胞(DF-1) Chicken lymphoma cell(DT40), chicken fibroblast(DF-1) | DROSHA,DICER,MBD3,KIAA1279,CDKN1B,EZH2,HIRA,TYRP1,STMN2,RET,DGCR | [ |
DF-1、鸡胚胎干细胞(ESCs) DF-1,chicken embryonic stem cells(ESCs) | C2EIP (2号染色体PGCs中表达) C2EIP (expression in PGCs on chromosome 2) | [ |
DF-1 | PPAR⁃γ (过氧化物酶体增殖物激活受体-γ)、ATP5E (ATP合成酶ε亚基)、OVA(卵清蛋白) PPAR⁃γ (peroxisome proliferator-activated receptor-γ),ATP5E (ATP synthase epsilon subunit),OVA(ovalbumin) | [ |
DF-1 | 肌肉生长抑制素Myostatin | [ |
鹌鹑肌肉细胞(QM7) Quail muscle cells(QM7) | MLPH (亲黑素位点) MLPH (melanophilin locus) | [ |
鸡原始生殖细胞(PGCs) Chicken primordial germ cells(PGCs) | CXCR4 (C-X-C趋化因子受体4型) CXCR4 (C-X-C chemokine receptor type 4) | [ |
ESCs | Stra8 (视黄酸激活基因8) Stra8 (stimulated by retinoic acid 8 gene) | [ |
DF-1 | GAPDH (甘油醛-3-磷酸脱氢酶) GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | [ |
DF-1 | TVA (抗A亚群禽白血病病毒受体)、TVC(抗C亚群禽白血病病毒受体)、TVJ(抗J亚群禽白血病病毒受体) TVA (anti A subgroup of avian leukemia virus receptor),TVC(anti C subgroup of avian leukemia virus receptor),TVJ(anti J subgroup of avian leukemia virus receptor) | [ |
DF-1 | TBK1 (TANK结合激酶1) TBK1 (TANK binding kinase 1) | [ |
DF-1 | DAZL (精症缺失样) DAZL (deleted in AZoospermia-like) | [ |
DF-1 | ANP32A (性核磷蛋白32A) ANP32A (acidic nuclear phosphoprotein 32 family,member A) | [ |
DF-1 | IRF7 (扰素调节因子7) IRF7(interferon regulator factor 7) | [ |
DF-1 | AMHR (缪勒管激素2型受体) AMHR (anti-mullerian hormone receptor type 2) | [ |
DF-1 | Apob (载脂蛋白B) Apob (apolipoprotein B) | [ |
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