Abstract:

The microflora in the frozen soil at the root of Saussureae Involucratae Kar. et Kir. et Maxim from Tianshan in Xinjiang Autonomous Region were isolated under different culture conditions and identified by 16S or 18S rDNA sequences amplification, the neighbor-joining phylogenetic tree was then constructed. The microflora producing all kinds of enzymes including proteinase, mannanasc, xylanase, cellulase and amylase etc, were screened by selective media. The results showed that rich enzyme-producing microflora existed in the frozen soil. Among 34 bacteria strains isolated, most of them mainly excreted amylase, and partly excreted cellulose degrading enzyme, such as mannanase, protein degrading enzyme and xylanase. The strains which excreted amylase are mainly in genus of Pseudeomonas, Flavobacteriu and Arthrobacter, while the bacteria that excreted cellulase belong to Pseudeomonas and Arthrobacter. Five fungi strains isolated from the frozen soil can produce amylase or xylanase.

Key words: Saussureae involucratae Kar. et Kir. et Maxim , frozen soil near root, enzyme-producing microflora

摘要：

利用不同培养基对新疆天山雪莲根部冻土微生物进行了分离与培养，通过16S或18SrDNA序列扩增初步鉴定了分离所得微生物，并构建系统发育树。结合选择性培养基进行了产蛋白酶、产甘露聚糖酶、产木聚糖酶、产纤维素酶和产淀粉酶等微生物的筛选。结果表明，新疆天山雪莲根部冻土中蕴含着丰富的产酶微生物，分离得到的34株细菌中大部分都能产酶，其中以产淀粉酶为主，部分产纤维素酶、甘露聚糖酶和木聚糖酶。产淀粉酶细菌主要属于Pseudeomonas，Flavobacterium和Arthrobacter等属，产纤维素酶细菌主要属于Pseudeomonas和Arthrobacter等属。分离得到的5株真菌，以产淀粉酶和木聚糖酶为主。

关键词: 雪莲 根部冻土 产酶微生物