Abstract:

Organophosphorus(OP) compounds which are widely used as high efficient and broad-spectrum insecticides caused broad-area pollution to environment.A bactrium with high efficient capability of degrading methyl parathion,identified as Ochrobactrum sp.by 16S rDNA analysis,is isolated from highly OP-polluted soil in this study.The full-length mphxw gene from this bactrium is cloned by PCR.The prokaryotic expression vector is constructed and the expressed products bore normal bioactivity in E.coli.The methyl para...

Key words: methyl parathion hydrolase, Ochrobactrum sp., gene cloning, gene expression;

摘要：

有机磷化合物作为一类高效广谱的杀虫剂被广泛使用,在使用的同时也造成了大面积的环境污染。本研究从被农药高度污染的土壤中筛选到了一株对甲基对硫磷农药有高效降解能力的细菌,通过16S rDNA鉴定为苍白杆菌(Ochrobactrum sp.)。克隆该菌的有机磷降解酶编码基因mphxw,构建原核表达载体,其表达产物具有正常生物学活性,同时纯化了重组酶。

关键词: 甲基对硫磷水解酶 苍白杆菌 基因克隆 基因表达