Table of Content

31 May 2008, Volume 10 Issue S1

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Molecular Mechanism of Abiotic Stress Signal Transduction

LI Hao-wen|ZHAO Jun

2008, 10(S1):  1-6. 

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Low temperature,drought and high salinity are main abiotic stresses which have adverse effects on plant growth and crop production.It is important to study the responses of plant to environmental stresses and the molecular mechanism of abiotic stress signal transduction.Recent research indicates that both ABA-dependent and ABA-independent regulatory systems are involved in stress-responsive gene expression.For several stress inducible genes,cis-acting elements in promoter regions and the corresponding trans...

Regulation of Nitric Oxide in Plant Drought Tolerance and Disease Resistance

LI Zao-xia|ZHAO Jun

2008, 10(S1):  7-11. 

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Nitric oxide(NO)as a signaling molecule plays a key role in plant physiological processes.This review mainly focuses on the NO synthesis in plant,its chemical properties,its functions as a signaling molecule,and the regulatory effects in plant drought tolerance and disease resistance.

Relationships between Aminopeptidase N (APN) and Bt resistance of Lepidoptera

MIAO Su-li1|ZHANG Shao-ping 2|CHENG Hong-mei 3

2008, 10(S1):  12-17. 

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The use of Cry toxins from Bacillus thuringiensis(Bt) to control insect pests is a revolutionary progress in crop production.Change in binding ability between Bt insecticidal proteins and its receptors may be the main mechanism of Lepidoptera insect resistance to Bt toxin.Aminopeptidase N(APN) is one kind of receptors for Bacillus thuringiensis(Bt) toxin in insect midguts.Through discussing its binding capability with Bt toxin,this paper summarizes the molecular biology mechanism of APN gene and Bt resistan..

Research Advances of Spectral Technology in Monitoring the Growth and Nutrition Information of Crop

SUN Jin-ying1|CAO Hong-xin2|HUANG Yun1

2008, 10(S1):  18-24. 

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With the development of spectral technology,the spectral analysis methods on plant have become an important means of monitoring the information of crop growth and nutrition.The research advances that monitor five indexes such as leaf area index(LAI),biomass,water content,chlorophyll content,and nitrogen content of crop growth and nutrition by the spectral technology were reviewed.The prospects of applying the spectral technology in monitoring the information of crop growth and nutrition were put forward.

The Advance in Low Potassium Tolerant Cotton Varieties

XU Xue|ZHANG Rui|GUO San-dui

2008, 10(S1):  25-29. 

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Potassium resources is lack in china and the potassium deficiency is common in cotton areas.Potassium is one of the most needed elements in cotton.Premature,yield reduction and red leaf blight can be caused by potassium deficiency.The research of low potassium resistant cotton is a matter as great urgency.Low potassium tolerant variety can be selected by conventional breeding.The potassium uptake genes have laid a basis for genetic engineering breeding,so the transgenic technology has been attractive.The im...

Progress in Research on Allelopathic Potential in an Exotic Invasive Plant,Eupatorium adenophorum

Guo Hui-ming|Cheng Hong-mei

2008, 10(S1):  30-34. 

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Eupatorium adenophora Sprengel(Asteraceae) is a noxious invasive alien weed,and it has become widespread in many countries and regions.In these invaded countries and regions,this weed has caused serious economic loss and threatened native biodiversity.Researchers have started their research extensively.In this paper,some aspects related to the allelopathy in Eupatorium adenophorum are summaried.

The Situation and Strategy of Cellulosic Ethanol Development

YU Ting|FU Yong-fu

2008, 10(S1):  35-40. 

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Cellulosic ethanol is a clean,resourceful and reproducible energy.Application of cellulosic ethanel will be one of the important ways to meet the challenges of energy safety,ecological protection and issues concerning agriculture,countryside and farmers in China.The current application of the bio-ethanol researches at home and abroad are summarized;the methods of increasing the yield of cellulosic ethanol by increasing the biomass and improving the quality of energy plants are put forward;the problems in th...

Development of Lignocellulosic Materials for Bioethanol Production

XING Qi-ming1|2|SUN Qi-zhong2|GAO Feng-qin2

2008, 10(S1):  41-45. 

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With global oil-prices rising,the future of biofuel made from plant materials become the focus of the society in all over the world.Grain is not suitable to be used for producing bioethanol because of shortage food and limited farmland.Lignocellulose is the most widely available biological material.Producing bioethanol by lignocellulose is an effective way to solve fule shortage.The theory and technology of lignocellulosic materials for bioethanol production were expounded in this paper.

Application of Shikimate and its Synthesis by Microbial Metabolism

GAO Jie1|2|XU Xiu-hong1|WAN Yu-song2|JIN Wu-jun2

2008, 10(S1):  46-51. 

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Shikimic acid is a valuable initial material used in pharmaceutics.Its derivates have been found to be involved in anti-inflammatory,anti-tumor,anti-cardiovascular diseases and against the avian influenza.The physicochemical property,pharmacologic characters and the production methods of the shikimate were reviewed in this paper.On the basis of recent progress at home and abroad,this paper focused on shikimate metabocic synthesis by microbe and methods for improving the production efficiency.

Protection and Construction of Farmland and Grassland Ecosystems in the Western Area of China

HE Wen-qing|YAN Chang-rong|LIU Qin|LIU Shuang

2008, 10(S1):  52-55. 

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Farmland and grassland are the main land utilization pattern in the western China.For resource sustainable development and utilization,it is urgent to strengthen the protection and construction of farmland and grassland resource.Comprehensive analysis on the status of farmland and grassland show that the resource of farmland and grassland are rich,but low productivity and deteriorative environment have been a serious drawback for the development of western China.The farmland and grassland is not only a guar...

Dynamic Variation of Soluble Sugar,Total Nitrogen and Malondialdehyde in Different Alfalfa Varieties and Their Effect on Alfalfa s Cold Resistance

TAO Ya1|2|SUN Qi-zhong2

2008, 10(S1):  56-60. 

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Six different alfalfa varieties at home and abroad were acted as materials.The dynamic changes of soluble sugar,total nitrogen and malondialdehyde was studied,so as to find out their relationships with cold resistance,and then variation analysis and cluster analysis were performed.The results indicated that the soluble sugar content increased from the middle of September and reached the peak in the middle of November,then descended gradually till the middle of May.It showed that soluble sugar was a kind of ...

The Application of the Plant Chloroplast trn Gene Universal Primers in Detection of Genetically Modified Plants and Their Derived Products

MA Yu-ting|WAN Yu-song|RUAN Hong-yue|HE Hui-qun|JIN Wu-jun

2008, 10(S1):  61-66. 

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Processed foods usually contain a variety of crops components,but the DNAs have been destroyed seriously,so it is difficult to do PCR detection for transgenic plants and their products.Using plant chloroplast trn gene which is of great amount copy numbers and widely exists in plants as a reference gene for transgenic plants and their products detection,the results show that the categories of different types of plants in processed foods can be identified only through one PCR reaction according to the size of...

Rapid and Semi-quantitative Detection of Pathogenic Vibrio paraharmolyticus from Environment and Aquatic Animals by PC-PCR

MA Yan1|2|LI Jian2|WANG Qun2|HE Yu-ying2|WANG Bin1

2008, 10(S1):  67-72. 

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A rapid and semi-quantitative method was developed for detection of pathogenic Vibrio paraharmolyticus,using tl gene as a targeted gene,combined the method of plate count for bacterial colonies and colony PCR.The expected PCR product was 673 bp.Detection limit for artificial contaminants was 3.1×102 cfu/g of solid sample and 1.3 102 cfu/ml of liquid sample;Detection limit of colony PCR reaction system was 90 cfu.The contaminated samples were detected by both PC-PCR and routine methods with a completely coin...

Study on the Water Conservation Properties of Environment Friendly Bast Fiber Mulch Film

FU Deng-qiang|YI Yong-jian|WANG Hong-ying|WANG Chao-yun

2008, 10(S1):  73-77. 

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Environment friendly bast fiber mulch film is regarded as one substitute for plastic mulch film and has a bright future in the sustainable development of agriculture,because it is a biodegradable and environment friendly product with high strength.Water conservation is one of the main functions of environment friendly bast fiber mulch film.This paper deals with its application performance in water conservation.The results indicate that environment friendly bast fiber mulch film mulching can increase rain ab...

The Positive Study on Innovative Capacity of China s Agricultural Science and Technology

LU Jiang-yong1|2|JIANG He-ping2

2008, 10(S1):  78-85. 

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The paper establishes the index system which evaluates the innovation capabilities of agricultural science and technology of China based on the characteristics of China s agricultural research system.And it employs the factor analysis method to do the empirical analysis about the innovation capabilities of agricultural science and technology of all provinces(districts/municipanities) of China in 2007 and ranked the results,which provides the scientific reference for the policymakers.

Stability of Phytase in Transgenic Corn in Simulated Gastric/ Intestinal Fluid

YANG Wen-zhu1|PU Ling-kui1|2|ZHANG Qi1|CHEN Ping1|CHEN Ru-mei1|FAN Yun-liu

2008, 10(S1):  86-89. 

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As one of the most important feedstuff enzymes,phytase is applied extensively worldwide.It s very important to get knowledge on phytase stablitity in gastrointestinal tract envionment for its application and function research.In vitro simulated gastric fluid(SGF) and simulated intestinal fluid(SIF) were established in this study.Samples treated by different time were carried on Western blotting analysis.Results showed that phytase was stable within 60 min in SGF and was stable within 15 min in SIF,indicatin...

Establishment and Optimization of Sliver Staining Differential Display in Cotton

AN Jun|TANG Qiao-Ling|WANG Miao|WANG Xu-Jing|WANG Zhi-Xing

2008, 10(S1):  90-93. 

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mRNA differential display technology is an effective method for screening differentially expressed genes.In this study,we optimized the silver staining differential display system in cotton.Total RNA were extracted from leaves and about 10 days DPA fiber of upland cotton Su 12.Differentially expressed cDNA fragments in fiber were selected by using differential display method.

Prokaryotic Expression and Sequence Identification of Chicken PEPT1Epitope Gene

JIANG Yong|CAI Hui-yi|LIU Guo-hua|LI Yong|ZHANG Shu

2008, 10(S1):  94-98. 

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According to the PEPT1 gene sequence in GenBank,specific primer sequences were designed to amplify the PEPT1 epitope fragment gene.The PEPT1 epitope gene was subcloned into expression vector pET-22b(+)to give pET-22b-PEPT1 and expressed in Rosetta(DE3).The expressed product was purified by Ni-NTA chromatography column and identified by SDS-PAGE,Western blot and mass spectrogram.This study was beneficial for further research on producing specific antibody for poultry intestinal PEPT1.

Cloning of a Methyl Parathion Hydrolase Gene from Ochrobactrum sp

XIAO Wei|CHU Xiao-yu|TIAN Jian|GUO Jun|WU Ning-feng

2008, 10(S1):  99-102. 

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Organophosphorus(OP) compounds which are widely used as high efficient and broad-spectrum insecticides caused broad-area pollution to environment.A bactrium with high efficient capability of degrading methyl parathion,identified as Ochrobactrum sp.by 16S rDNA analysis,is isolated from highly OP-polluted soil in this study.The full-length mphxw gene from this bactrium is cloned by PCR.The prokaryotic expression vector is constructed and the expressed products bore normal bioactivity in E.coli.The methyl para...

The Effect of Bio-additives on Mixed Silage Quality of Sugar Beet and Corn stalks

LI Xiao-xia1|Wang Zong-li2|SUN Qi-zhong2

2008, 10(S1):  103-106. 

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The effect of different biological additives(LF、Caihe、Tianzha、LF+Caihe、Tianzha+ Caihe) on mixed silage quality of sugar beet and corn stalks was studied.When any additives was added,pH,the content of ADF,NDF and CF of silage reduced significantly,and the CP of silage increased.All kinds of additives could improve fermentation quality of silage.Among them,Caihe was the optimal additive.

Effect of Different Density on Yield of Corn Variety Zhengdan136

ZHOU Bo1|HU Xue-an1|WEI Liang-ming1|XUE Hua-zheng1|WU Guo-qiang2|HUANG ling

2008, 10(S1):  107-110. 

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The field experiments of different density of new corn variety Zhengdan136 were developed at Kaifeng city in 2007.Comparison analysis were carried outon the growing time,plant height,the trait of ear and yield components under different planting density.The results showed that the optimum density of the new corn variety Zhengdan136 is 75000 plants/hm2.The test gave some scientific basis for the popularizing this variety in a larger scale of Henan province.

Comparison and Improvement of RNA Purification Methods in Rice

LIU Ling|XIE Jia-jian|WANG Xi-feng|PENG Yu-fa

2008, 10(S1):  111-115. 

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Total RNA was extracted from rice leaves using TRIZOL regent.Three commonly used RNA purification methods and an improved method were compared in this experiment.The quality of total RNA was analyzed through gel electrophoresis,UV spectrophotometry and RT-PCR.The RNA purified by the improved method gave clear bands of rRNA in the electrophoresis without DNA contamination and degradation.OD260/OD280 value was from 1.91 to 1.98.RNA purified by the improved method was used for RT-PCR and none band of DNA conta...

Preparation of HMW DNA for Streptomyces albulus var. wuyiensis and Determination of the Optimum Conditions for Restriction Enzyme Digestion

WANG Wan-qun1|A-la-tan-fu1|2|ZHAN Li-ran1|3|SHI Yi-pin1|ZHANG Ke-cheng1

2008, 10(S1):  116-119. 

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In order to construct bacterial artificial chromosomes library of Streptomyces albulus var.wuyiensis,we extractd high molecular weight DNA(HMW-DNA) from Streptomyces albulus var.wuyiensis.Mycelium pellet of Streptomyces albulus var.wuyiensis was embeded in low melting point agarose.The agarose block was treated by lysozyme,proteinase K and restriction enzyme.Contour-clamped homogeneous electric field(CHEF) gel electrophoresis was performed for prepared DNA plugs.Electrophoresis analysis shows no degradation...