Abstract:

As one of the most important feedstuff enzymes,phytase is applied extensively worldwide.It s very important to get knowledge on phytase stablitity in gastrointestinal tract envionment for its application and function research.In vitro simulated gastric fluid(SGF) and simulated intestinal fluid(SIF) were established in this study.Samples treated by different time were carried on Western blotting analysis.Results showed that phytase was stable within 60 min in SGF and was stable within 15 min in SIF,indicatin...

Key words: phytase, transgenic corn, simulated gastric/intestinal fluid, stability;

摘要：

植酸酶作为应用最为广泛的重要饲料酶制剂之一,明确其在动物消化道环境中的稳定性对于其应用及作用方式的研究具有重要意义。本研究在体外建立了胃、肠两种模拟消化体系,取不同消化时间的样品进行Western杂交,结果显示在模拟胃液中植酸酶蛋白经60 min处理未被消化,而在模拟肠液中处理15 min内被全部消化,表明转植酸酶玉米种子中的植酸酶蛋白在模拟胃液中极难被消化,可稳定存在,在模拟肠液中可被消化。

关键词: 植酸酶 转基因玉米 模拟胃/肠液 稳定性