Abstract:

A rapid and semi-quantitative method was developed for detection of pathogenic Vibrio paraharmolyticus,using tl gene as a targeted gene,combined the method of plate count for bacterial colonies and colony PCR.The expected PCR product was 673 bp.Detection limit for artificial contaminants was 3.1×102 cfu/g of solid sample and 1.3 102 cfu/ml of liquid sample;Detection limit of colony PCR reaction system was 90 cfu.The contaminated samples were detected by both PC-PCR and routine methods with a completely coin...

Key words: Vibrio parahaemolyticus, rapid detection, semi-quantitative, colony PCR, tl gene;

摘要：

以tl为靶基因,将菌落PCR技术和平板菌落计数法相结合,建立致病性副溶血弧菌快速、半定量检测方法。PCR扩增的目的片段为673 bp,人工污染样品检测最低限固体样品为3.1×102cfu/g,液体样品为1.3×102cfu/mL,菌落PCR每反应体系的检测低限为90 cfu。对人工污染样品的检测结果显示,PC-PCR方法检测结果与常规检测结果一致,可用于养殖环境及水产品中细菌的动态监测。

关键词: 副溶血弧菌 快速检测 半定量 菌落PCR tl基因