Abstract:

Delftia tsuruhatensis AID and Acinetobacter calcoaceticus PHEA-2 can utilize aniline and phenol as the sole carbon source, respectively. Catechol is the first mid-metabolites through the degradation of aromatic compounds, which is degrade by certain enzymes to TCA-cycle intermediates. A complete aniline dioxygenase gene from AID strain was cloned into a broad host range plasmid, the resulting plasmid pVD was introduced by triparental mating into wildtype strain AD9 and a phenol-degrading bacterium, A. calcoaceticus PHEA-2, respectively. The over-expression of aniline dioxygenase gene enhanced the aniline degradation of AD9 strain and conferred the capable of aniline utilization to the PHEA-2 strain.

Key words: aniline dioxygenase, Delftia tsuruhatensis AD9, Acinetobacter calcoaceticus PHEA-2

摘要：

Delftia tsuruhatensis AD9和Acinetobacter calcoaceticus PHEA-2分别能利用苯胺和苯酚作为唯一碳源生长，降解的第一个中间产物均为邻苯二酚，随后裂解为参与三羧酸循环的中间产物。通过PCR方法克隆到苯胺高效降解菌AD9的苯胺双加氧酶基因，并构建表达苯胺双加氧酶的广宿主质粒载体pVD，通过三亲接合，导入到AD9和PHEA-2中。对两种重组菌中苯胺双氧酶基因的表达及苯胺降解特性的分析结果表明，增加苯胺双加氧酶基因的拷贝数可以提高野生型AD9的苯胺降解速率，同时该基因的表达使PHEA-2菌获得苯瞎隆觎能寸1．

关键词: 苯胺双加氧酶 Delftia tsuruhatensis AD9 Acinetobacter calcoaceticus PHEA-2