Abstract:

In this study, reference molecules suitable for event-specific qualitative detection of genetically modified (GM) maize Bt11,  TC1507, T25 and GA21, respectively, were constructed and validated by inter-laboratory validation. The statistical analysis  of reported data from seven labs showed that pBt11, pTC, pT25 and pGA were highly specific to GM maize Bt11, TC1507, T25 and GA21, respectively. The limits of detection that could be detected by all participants were all 50 copies DNA of each reference molecule for the detection of zSSⅡb and event-specific sequence in qualitative PCR detection systems. All results indicated that the four constructed reference molecules can be used as reference materials for qualitative event-specific detection of the corresponding GM maize events.

Key words: reference molecule, event-specific, inter-laboratory trail, genetically modified maize

摘要：

构建了分别适于转基因玉米GA21、TC1507、T25和Bt11品系特异性双重PCR检测标准分子,并对其在定性PCR检测体系中的适用性进行了实验室间协同实验验证。对7家实验室返回结果的统计分析表明,标准分子pBt11、pTC、pT25和pGA分别对转基因玉米Bt11、TC1507、T25和GA21品系具有高特异性。4个标准分子在zSSⅡb和对应品系特异性序列单一PCR检测体系中检测下限均为50拷贝。因此,4个标准分子均可作为新型标准物质用于相应转基因玉米品系特异性定性检测。

关键词: 标准分子;品系特异性;协同实验;转基因玉米