Toxic fungi is easy to contaminate food and produce mycotoxins， especially in the growth and processing of cereal， among which aflatoxin is more harmful to human body， seriously endangering the health of consumers. Efficient food safety detection technology is important means to protect consumers from aflatoxin poisoning. This paper introduced the research progress of aflatoxin detection in food at home and abroad in recent years. The application and existing problems of high performance liquid chromatography（HPLC）， liquid chromatography-mass spectrometry（LC-MS）， enzyme-linked immunosorbent assay（ELISA）， time-resolved fluorescence immunoassay（TRFIA）， gold immune chromatography assay（GICT）， surface enhanced Raman spectroscopy （SERS） and electrochemical sensor（ES） in the detection of aflatoxin in food were analyzed， so as to provide reference for related research.