›› 2014, Vol. 16 ›› Issue (1): 65-70.DOI: 10.13304/j.nykjdb.2013.152

• 生物技术 生命科学 • 上一篇    下一篇

玉米细菌性茎腐病组织中一株新的铜绿假单胞杆菌的分离鉴定

杨丽萍1,2,杨根华1,李枝林1,谢华2*,马荣才2*   

  1. (1.云南农业大学园林园艺学院, 昆明 650201; 2. 北京市农林科学院农业生物技术研究中心, 北京 100097)
  • 出版日期:2014-02-15 发布日期:2014-02-17
  • 通讯作者: 谢华,副研究员,博士,主要从事植物软腐病的研究。E\|mail:xiehua@baafs.net.cn; 马荣才,研究员,博士,主要从事植物软腐病的研究。E\|mail:marc@biny.gov.cn
  • 作者简介:杨丽萍|硕士研究生|研究方向为植物病理学。E\|mail:xxyangliping@163.com。
  • 基金资助:

    国家转基因生物新品种培育重大专项(2009ZX08003)资助。

Isolation and Characterization of a New Pseudomonas aeruginosa Strain from Bacterial Stalk Rotted Maize

YANG Li\|ping1,2, YANG Gen\|hua1, LI Zhi\|lin1, XIE Hua2*, MA Rong\|cai2*   

  1. (1.Horticulture College, Yunnan Agricultural University, Kunming 650201|2.Beijing Agro\|Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, China)
  • Online:2014-02-15 Published:2014-02-17

摘要:

为了明确导致河南省郑州市玉米细菌性茎腐病的病原菌,对从玉米细菌性茎腐病样品中分离得到的病原菌进行了纯化培养、形态学观察、致病性测定、生理生化特征分析和16S rDNA的遗传关系分析等研究。结果表明,所分离的菌株(YM02)为革兰氏阴性致病菌,能利用多种糖(醇)化合物产酸,但可使阿拉伯半乳聚糖产碱。YM02与国内外分离的铜绿假单胞杆菌(Pseudomonas aeruginosa)菌株16S rDNA的序列相似性均在99%以上,故将其命名为Pseudomonas aeruginosa菌株YM02,该菌与Pseudomonas aeruginosa菌株ANSC、S164S、S167S(2)和CS\|2菌株遗传关系最近,该病原菌的分离和鉴定为玉米细菌性茎腐病的防治提供了基础资料。

关键词: 玉米, 细菌性茎腐病;铜绿假单胞杆菌

Abstract:

To identify the pathogen of maize bacterial stalk rot disease occurred in Zhengzhou, Henan Province, the strain YM02 was isolated from maize stalk rot samples and then identified and characterized by morphology observation, pathogenicity test, biochemical and physiological characteristics analyses, and molecular phylogeny genetic relationship analysis. The results showed that the isolated strain (YM02) was a Gram\|negative pathogenic bacterium; it could ferment several kinds of sugar or alcohol to produce acid, and transfer arabinogalactan into alkali. In addition, the 16S rDNA sequence of YM02 strain had a similarity of above 99% with those of internal and external isolated Pseudomonas aeruginosa strains. Thus, this strain was named as Pseudomonas aeruginosa YM02, which had the closest genetic relationship with Pseudomonas aeruginosa strains ANSC、S164S、S167S(2) and strains CS\|2. The isolation and characterization of this pathogen had provided basic data for the prevention and treatment of maize bacterial stalk rot disease.

Key words: maize, bacterial stalk rot disease, Pseudomonas aeruginosa

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