关键词: 玉米;茉莉酸;转录调控;虫害诱导启动子

Abstract:

Upon being attacked by herbivores, the plant defense response is triggered, which often involves complex transcriptional regulation of the defense genes. This process depends on a plethora of defense-related transcription factors and herbivory-inducible cis-elements on the promoters of defense genes, and is largely modulated by the jasmonate pathway. Current studies on these cis-elements focus on the model dicot Arabidopsis, and it is unknown whether they are also conserved in monocot crops. In order to explore the answer, this paper characterized the activity of 2 jasmonate- and herbivory-inducible maize promoters in Arabidopsis. It was found that these 2 promoters could not drive GUS expression in untreated Arabidopsis, but strong GUS expression could be detected in various tissues, when plants were treated with jasmonate. These results suggested that the expressive gene induced after being bitten by insect was at the same time induced by JA. This result would lay a foundation for studying the response molecular mechanism of JA induced herbivore.

Key words: maize, jasmonate, transcriptional regulation, herbivore-induced promoter