关键词: 水稻(Oryza sativa L.), 卷叶突变体, T-DNA, 光合效率

Abstract: Leaf is a main organ for plant photosynthesis. Fine leaf shape is beneficial for building an ideal plant type and improving the efficiency of photosynthesis. In order to study the molecular mechanism of leaf morphogenesis, an half rolled leaf mutant rlm1 was discovered from the rice T-DNA insertion mutant library. The mature leaf of rlm1 mutant involuted along midrib, and finally become half cylinder. The leaf rolling index was 0.64, leaf erect parameter was 95, and the mutant photosynthesis efficiency was higher than the wild one. The mutation site of rlm1 was verified by mapping at the 1st intron of gene LOC_Os03g06654, which encoded flavin-containing monooxygenase. RT-PCR expresion and analysis indicated that LOC_Os03g06654 was inactive completely by T-DNA insertion. The gene was another allele of the reported rice curly leaf gene OsCOW1(Constitutively Wilted 1). Due to the good agronomic property of rlm1, it could be used to conduct high photosynthetic breeding practice.

Key words: rice(Oryza sativa L.), rolled leaf mutant, T-DNA, photosynthesis efficiency