关键词: 水稻, 卷叶突变体, 遗传分析, 图位克隆

Abstract: Leaf is the main organ of plant photosynthesis, fine leaf shape is beneficial for shaping the ideal plant type and improve the efficiency of photosynthesis. In order to study the molecular mechanism of leaf morphogenesis, an extreme rolled leaf mutant zw209 was screened from the rice T-DNA insertion mutant library. Compared with the wild type, zw209 had two significant features: (1) the number and area of mutants bulliform cells were smaller; (2) the chlorophyll content and photosynthetic efficiency of mutant were higher. Genetic analysis showed that the mutant trait was controlled by a single recessive nuclear gene. Through map-based cloning, the gene ZW209 was located on the long arm of rice chromosome 9 and a 92.3 kb region between markers InDel136 and InDel140. There was no rolling leaf rice gene had been reported in this section, so the gene ZW209 might be a new gene loci.  Above results defined the inheritance and phenotype characteristics of this inward rolled leaf mutant, which provided a basis for cloning this gene and functional mechanism analysis.

Key words: rice, rolled leaf mutant, genetic analysis, map-based cloning