关键词: 植物组织培养, 间歇浸没生物反应器, 铁皮石斛, 增殖率

Abstract: At present, Dendrobium officinale seedlings are mainly produced by plant tissue culture. Solid culture method is characterized by high production costs and low multiplication rates, which is unable to satisfy the increasing market demands. Temporary immersion bioreactors（TIB）can reduce labor and costs of production. This study compared the multiplication rates, plant height, diameter of stem, root system growth and survival rates after transplantation of D. officinale plantlets cultured in self-developed TIB and solid medium. The multiplication rates and quality of plantlets produced in TIB were significantly higher than those produced in solid medium. Plantlets produced in TIB with moderate root size were suitable for cultivation, and their survival rates after transplantation were significantly higher than those produced in solid medium. These results showed that TIB provided a new protocol for production of D. officinale seedlings.

Key words: plant tissue culture, temporary immersion bioreactors, Dendrobium officinale, multiplication rates