关键词: 金钗石斛, 植物组织培养, 植物生物反应器, 浸没频率

Abstract: Taking the protocorm of Dendrobium nobile Lindl. as material, the tissue culture and rapid propagation of D. nobile were studied in the temporary immersion bioreactor. The optimum culture conditions were obtained: the immersion frequency was 5 min / 8 h, the inoculation amount was 10 g·L-1 and the sucrose concentration was 20 g·L-1. Compared with the traditional solid culture, the bioreactor culture was superior to the solid culture in the growth morphology, multiplication rate and effective components of the tissue culture seedlings, which provided a new method for the production of D. nobile seedlings with high efficiency and low cost.

Key words: Dendrobium nobile Lindl., plant tissue culture, plant bioreactor, immersion frequency