中国农业科技导报 ›› 2016, Vol. 18 ›› Issue (5): 54-61.DOI: 10.13304/j.nykjdb.2016.065

• 生物技术 生命科学 • 上一篇    下一篇

拟南芥NUP107-160亚复合物突变体表型分析

肖龙1,刘文瑄2,陈福禄3,张晓玫3,陈庆山1*,傅永福3*#br#   

  1. 1.东北农业大学农学院, 哈尔滨150030; 2.北京林业大学生物科学与技术学院, 北京 100083;
    3.中国农业科学院作物科学研究所, 农业部北京大豆生物学重点实验室, 北京 100081
  • 收稿日期:2016-01-28 出版日期:2016-10-15 发布日期:2016-03-15
  • 通讯作者: 傅永福,研究员,博士生导师,主要从事植物发育生物学研究。E-mail:fuyongfu@caas.cn;陈庆山,教授,博士生导师,主要从事大豆分子遗传育种研究。E-mail:qshchen@126.com
  • 作者简介:肖龙,硕士研究生,研究方向为植物发育生物学。E-mail:xiaolong90523@163.com;
  • 基金资助:
    国家自然科学基金项目(31370324,30670189和31571411)资助。

The Phenotype Analysis of NUP107-160 Subcomplex Mutants in Arabidopsis

XIAO Long1, LIU Wen-xuan2, CHEN Fu-lu3, ZHANG Xiao-mei3, #br# CHEN Qing-shan1*, FU Yong-fu3*   

  1. 1.College of Agriculture, Northeast Agricultural University, Harbin 150030; 2.College of Biological Science and Technology,
    Beijing Forestry University, Beijing 100083; 3.Beijing Key Laboratory of Soybean Biology, Ministry of Agriculture;
    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-01-28 Online:2016-10-15 Published:2016-03-15
  • Supported by:
    nuclear pore complex; NUP107-160 subcomplex; nucleoporin; circadian clock; root; flowering; Arabidopsis

摘要: 核孔复合物(NPC)是真核细胞核膜上比较大的蛋白复合物之一,它通过调节核质间的物质运输,参与生物的多种生长发育过程。目前,植物中只有少数NPC组分的功能得到了初步分析。通过对NUP107-160亚复合物中各组分突变体的鉴定,发现nup160、nup96、nup85、nup107和seh1突变体的叶片振动周期延长,主根的伸长受到抑制,其中nup160、nup96和nup107三种突变体在长日照条件下开花时间显著提前。结果表明NUP107-160亚复合物可能参与拟南芥生物节律、根生长和开花时间的调节。

关键词: 核孔复合物, NUP107-160亚复合物, 核孔蛋白, 生物钟, 根, 开花, 拟南芥

Abstract: Nuclear pore complex (NPC) is one of the large protein complex in nuclear envelope, and is involved in lots of growth and developmental processes through gating nucleocytoplasmic trafficking of RNA and proteins in various organisms. Currently, only several components function of NPC have been  analyzed in plants. Though identifying the mutants of all members in NUP107-160 subcomplex and analyzing their phenotypes, this paper found that mutants of nup160, nup96, nup85, nup107, and seh1 had longer circadian periods of leaf movement and shorter primary roots. But, only three mutants, nup160, nup96, and nup107,  significantly displayed early flowering. The results indicated that NUP107-160 subcomplex might be a novel player for the regulation of circadian clock, root elongation, and flowering time in Arabidopsis.