关键词: NDR1, 转基因水稻, 白叶枯病, 抗性鉴定

Abstract: NDR1(non-race-specific disease resistance 1) gene can mediate broad-spectrum in plant disease resistance and plays an important role in defense responses. Two  NDR1 homologous genes CaNDR1a and CaNDR1b were cloned from Chenopodium amaranticolor, and plant expression vectors  were constructed and generated transgenic rice by agrobacteria -mediated transformation, respectively. PCR and Southern blot assay demonstrated that those two genes were integrated into rice genome. Parts of transgenic lines were selected to conduct identification of resistance to rice bacterial leaf blight. The length of leaf disease spot were recorded in 3 d, 5 d, 10 d  after inoculation. Results indicated that the expression of CaNDR1a and CaNDR1b in rice had certain resistance to bacterial leaf blight and delayed symptom development of the pathogen. Expression levels of exogenous genes in transgenic rice plants were significantly higher than those of control. However, higher disease resistance line did not show the highest expression results of CaNDR1. There were not significant different with receptor rice in agronomic traits such as seed setting rate and thousand seed weight, besides of shorter plant.

Key words: NDR1, transgenic rice, bacterial leaf blight, disease resistance