关键词: 翻译速率, 重组蛋白, 表达量

Abstract: Using genetic engineering technology to express and obtain high-yield recombinant proteins plays an important role in the development of modern biology and application. Studies have found that heterologous expression of original genes was very difficult. After the expression system was modified, such as optimizing of promoter, signal peptide and increasing gene copies, the quantity of recombinant protein expression was still very low. Therefore, exploring new way to increase the gene expression is a hot research topic. In order to get more effective strategies to increase recombinant protein expression level,  this article comprehensively narrated the effected on the translation rate of protein based on the translation initiation area, codon preferences, mRNA secondary structure and SD-like sequence.

Key words:
translation rate, recombinant protein, expression quantity