关键词: 人肿瘤坏死因子相关凋亡诱导配体, 本氏烟, 瞬时表达, 表达量, 生物学活性

Abstract:

Human tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced apoptosis exhibits selective cytotoxicity, i.e. selectively induces apoptosis in numerous tumor cell lines while sparing normal cells, thus it has been regard as a promising candidate medicine of apoptosis-inducing therapy in malignancies. Plant bioreactor has been widely concerned due to its advantages in production of pharmaceutical proteins. Expression vector of soluble human TRAIL (shTRAIL) gene was constructed, and the Nicotiana benthamiana transient expression system was utilized in this paper. The results showed that the mean expression level of shTRAIL was 68.60 pg/mg TSP in N. benthamiana leaves, and the inhibition rate of NCI-H460 was 28.75% when treated with 200 pg/mL shTRAIL. This results indicated that the codon optimized gene could express target protein with biological activity in transgenic plants, and provide a basis for next stage of stable transformation of the target gene.

Key words: tumor necrosis factor related apoptosis-inducing ligand(TRAIL), Nicotiana benthamiana, transient expression, expression level, biological activity