关键词: 甜高粱, 成熟种子, 离体再生

Abstract: Sweet sorghum is one of the most advantageous biomass energy crops. In order to improve the transgenic breeding efficiency of sweet sorghum, this paper took the matured sweet sorghum (Sorghum bicolor L. Moench) seeds as explants, analyzed the factors affecting callus formulation, regeneration in vitro, and established a technology system of using matured sweet sorghum seeds for regeneration in vitro. The results showed that treating sweet sorghum seeds with 0.1% mercuric chloride for 10 min could gain lower pollution rate (20%) and higher germination rate (76.7%). Inoculated the seeds of sweet sorghum variety Yajin 4184 on MSB5 basic medium with concentration of 3.0 mg/L 2,4-D and 0.3 mg/L 6-BA for 3 weeks could obtain the highest callus induction rate of 81.7%. The highest budding rate was 93.3%, when callus was cultured on B5 medium adding 0.2 mg/L NAA and 1.6 mg/L KT for 4 weeks. The indefinite buds cultured on 1×MS medium with 2.0 mg/L IBA for 3 weeks could reach the maximal rooting rate (96.7%). The establishment of a high frequency and high efficiency system for sweet sorghum regeneration in vitro would provide convenient method for improving sweet sorghum hereditary features and speed up the breeding process of sweet sorghum.

Key words: sweet sorghum, matured seed, regeneration in vitro