中国农业科技导报 ›› 2018, Vol. 20 ›› Issue (2): 86-92.DOI: 10.13304/j.nykjdb.2017.0502

• 食品与营养 • 上一篇    下一篇

不同条件下酶法测定蜂蜜中甘油含量的研究

李亮1,孙萍1,宁准梅2,纪沛婧1,贾英杰1*   

  1. 1.上海冠生园蜂制品有限公司, 上海 201419; 2.上海工微所科技有限公司, 上海 200233
  • 收稿日期:2017-08-07 出版日期:2018-02-15 发布日期:2017-11-21
  • 通讯作者: 贾英杰,工程师,研究方向为蜂产品质量安全与分析。E-mail:loconey@sina.com
  • 作者简介:李亮,工程师,硕士,研究方向为蜂产品质量安全与分析。E-mail:chayeliliang@163.com。
  • 基金资助:
    科技部农业科技成果转化资金项目(2014GB2C000070)资助。

Determination of Glycerol Content in Honey by Enzymatic Method under Different Conditions

LI Liang1, SUN Ping1, NING Zhunmei2, JI Peijing1, JIA Yingjie1*   

  1. 1.Shanghai Guanshengyuan Bee-product Co., Ltd., Shanghai 201419;2.Shanghai Industrial Microbiology Institute Technology Co., Ltd., Shanghai 200233, China
  • Received:2017-08-07 Online:2018-02-15 Published:2017-11-21

摘要: 为了探究在不同条件下蜂蜜中的甘油含量,通过甘油酶制剂法测定其含量,实验结果表明,利用甘油酶制剂法能较稳定的测定蜂蜜中的甘油含量,其加标回收率实验的回收率在95.7%~115.0%之间,精密度实验甘油含量的RSD在2%~7%,重复性实验甘油含量的RSD在1%~5%;同时pH为3.00~7.00时,蜂蜜中的甘油含量变化较稳定,在7 d内,通过此法测定蜂蜜中甘油的含量无明显变化,在4~25℃条件下,蜂蜜中甘油含量变化较稳定。同时采用此法对不同蜂蜜中的甘油含量进行测定,能够达到生产上基本测定的要求。

关键词: 蜂蜜, 酶制剂, 甘油

Abstract: To explore the content of glycerol in honey under different conditions, this experiment determined the content of glycerol by glycerol enzyme method. The results showed that the glycerol enzyme preparation method could be used to determine the content of glycerol in honey. The recovery rate of oil content in this experiment was 95.7%~115.0%. The glycerol content of RSD was 2%~7% in the precision test and the glycerol content of RSD was 1%~5% in the repeatability test. In addition, the results was independent of pH when its level varied in the range of 3.00~7.00. Moreover, the content of glycerol in honey presented tiny variation by this method within 7 d. The content of glycerol in honey was stable under 4~25℃. Thus, this method could be used to determine the content of glycerol in different honey, and meet the requirements of basic determination in practical production.

Key words: honey, enzymic preparation, glycerol