关键词: 玉米, 细胞质雄性不育, 花粉, 线粒体, 杂种优势

Abstract: Cytoplasm male sterility (CMS) has widely been applied in agricultural breeding. Maize CMS lines have already played and will be continuously playing an indispensable and important role in new maize variety breeding and maize industrialization. This study discovered a cytoplasmic male-sterile line named CF3 developed by crossing of 2 maize inbred lines C7-2 and PH4CV. Furthermore, the derived sterile line CZ58 was obtained. Phenotype investigation and cytolog observation showed that the anthers of CMS lines, CF3 and CZ58 were not exposed. CF3 and CZ58 could generate a little bits of pollens. However, their pollen presented irregular translucent, and had no activity. Results of both PCR and sequencing showed that CF3 and CZ58 were novel CMS-S types. Genetics analysis suggested that the tassel presented clear infertility and stability. CF3 and CZ58 could be restored by various inbred lines, indicating that CF3 was a new S type cytoplasmic male-sterile line. CF3 and its derived line CZ58 had better applying potential in maize cross breeding and extension.

Key words: maize, cytoplasm male sterility (CMS), pollen, mitochondria, hybrid vigor