关键词: 沙蚕, 蛋白酶, 肽分子量, 抗氧化

Abstract: Marine biological protein resources are  important parts of marine living resources. High-value processing and utilization of marine biological resources is important for marine biological technology research. Enzymatic hydrolysis is an effective way to improve the function of marine proteins. However, different types of enzymes have different action sites, which may have certain effects on the activity of enzymatic hydrolysis products. In order to explore the antioxidant properties of different enzymatic hydrolysis products of Nereis succinea, 6 protease (alkaline protease, neutral protease, pepsin, papain, flavor protease, trypsin) were used to enzyme Nereis succinea, and some indexes reflecting the level of antioxidant, such as the degree of hydrolysis, molecular weight distribution and the scavenging rate of 3 kinds of free radicals (DPPH, O-2·, ·OH) were measured. The results showed that trypsin had the highest yield of small molecule peptides at 70.47%, and the pepsin was second with 68.12%. At the concentration of 5 mg·mL-1, pepsin and papain had better effects on DPPH scavenging rate of 90.87% and 90.52% after 4 h enzymatic hydrolysis, respectively.  The best effect on O-2· scavenging rate was 89.78% after 3 h pepsin hydrolysis. Papain had the second best effect on O-2· scavenging rate after 3 h enzymatic hydrolysis, which was 57.99%. The effects of alkaline protease hydrolysis for 4 h and pepsin hydrolysis for 2 h on the ·OH scavenging rate were good, which were 85.07% and 83.37%, respectively. Trypsin and pepsin had obvious effects on improving the hydrolysis degree of enzymatic hydrolysate and the production of small peptide, and pepsin hydrolysis had better scavenging rate for 3 kinds of free radicals, which indicated that pepsin was more suitable for the preparation of antioxidation peptide from Nereis succinea.

Key words: Nereis succinea, protease, peptide molecular weight, antioxidation