关键词: 转基因烟草, 雪花莲凝集素, 苋菜凝集素, 蚜虫抗性

Abstract: Aphid is a common crop pest. As a kind of agricultural pest and the carrier of plant viruses, aphid results in serious damage in the development of agriculture and its control has always been a difficult problem faced by many scientists. A bivalent plant expression vector PB1121-GA containing the agglutinin genes of Glanthus nivalis and Amaranthus caudatus was constructed and transformed into tobacco by using Agrobacterium-mediated disc method. 65 KanR transgenic tobacco plants were regenerated. Then 5 transgenic tobacco plants expressing different levels of agglutinin were selected from these KanR plants through genomic PCR, ELISA, RT-PCR and Western-blot. A preliminary aphid bioassay experiment was carried out for investigation of the transgenic tobacco resistance to Myzus persicae, which showed that the aphid inhibition rate was significant, with the maximum reaching to 85.8%. Above results provided a theoretical basis for the research on breeding of crops resistant to aphid and the application of bivalent aphid resistance genes.

Key words: transgenic tobacco, Glanthus nivalis agglutinin, Amaranthus caudatus agglutinin, aphid resistance