不同成熟期烤烟主脉中膜脂过氧化及其与衰老相关基因的关系探究

doi:10.13304/j.nykjdb.2019.0835

中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (3): 66-72.DOI: 10.13304/j.nykjdb.2019.0835

不同成熟期烤烟主脉中膜脂过氧化及其与衰老相关基因的关系探究

范宁波1,周俊学2,江凯2,王宏2,史龙飞2,高玉龙3*,陈颐3*

1.河南农业大学烟草学院, 郑州 450002;
2.河南省烟草公司洛阳市公司, 河南洛阳 471000;
3.云南省烟草农业科学研究院, 昆明 650031

收稿日期:2019-10-11 出版日期:2021-03-15 发布日期:2019-11-20
通讯作者: 高玉龙 E-mail：gyl3000@163.com；陈颐 E-mail：229781579@qq.com
作者简介:范宁波 E-mail：13643812806@163.com
基金资助:
云南省应用基础面上项目(2017FB074, 2018BB019)；
云南省烟草专卖局科技项目(2017YN05, 2018530000241017, 2018530000241027, 2018530000241022, 2019530000241019)；
云南省技术创新人才项目（2019HB068）；
云南省万人计划青年拔尖人才项目（2018-73）

Membrane Lipid Peroxidation and Its Relationship with Senescence-Related Genes in Main Veins of Flue-Cured Tobacco at Different Maturity Stages#br#

FAN Ningbo1, ZHOU Junxue2, JIANG Kai2, WANG Hong2, SHI Longfei2, GAO Yulong3*, CHEN Yi3*

1.Tobacco College, Henan Agricultural University, Zhengzhou 450002, China;
2.Luoyang Company, Henan Tobacco Company, Henan Luoyang 471000, China;
3.Yunnan Institute of Tobacco Agricultural Sciences, Kunming 650031, China

Received:2019-10-11 Online:2021-03-15 Published:2019-11-20

摘要/Abstract

摘要： 为了探究烟叶成熟和衰老过程中其主脉中的膜脂过氧化规律，明确主脉衰老机理，以烤烟品种‘K326’和‘豫烟6号’为试验材料，测定分析烤烟主脉中活性氧物质（O^2- 、H₂O₂）和膜脂代谢产物丙二醛（malondialdehyde，MDA）含量及衰老相关基因（NtCP1、NtCP2）的表达，并结合NBT染色结果来分析烤烟主脉的成熟衰老机理。结果表明，烤烟主脉中的活性氧物质和MDA含量在烟叶的成熟过程中的变化趋势基本一致，均随着烟叶成熟度的增加而逐渐上升。随着烤烟成熟衰老的推进，NtCP1基因表达量逐渐上升，NtCP2基因的表达量呈先上升后下降的趋势，直至低于未熟时期。相关分析表明，O^2- 、H₂O₂和MDA含量之间的相关性较高，且NtCP1表达量与O^2- 、H₂O₂和MDA含量之间呈极显著正相关关系，NtCP2表达量与O^2- 、H₂O₂和MDA含量之间呈负相关关系。综上所述，在烟叶成熟过程中，其主脉中的活性氧物质和MDA是逐渐积累的，衰老相关基因的表达与烟叶的成熟度也密切相关。研究结果为烟叶成熟过程中主脉的氧化衰老规律研究提供理论依据。

关键词: 烤烟, 主脉, 成熟度, 活性氧, 基因表达

Abstract: In order to investigate the law of membrane lipid peroxidation in the main veins of flue-cured tobacco during maturation, and to clarify the mechanism of aging of main veins, flue-cured tobacco varieties ‘K326’ and ‘Yuyan 6’ were used as experimental materials to determine the content of reactive oxygen species (O^2-, H₂O₂), malondialdehyde (MDA) and senescence-related genes (NtCP1, NtCP2) expression, and combined with NBT staining results to reveal the ripening and scenescence mechanism of main veins of flue-cured tobacco. The results showed that the variation trends of the content of active oxygen and MDA in the main vein were basically same, all increased with the maturity of tobacco. With the development of maturity, the expression of NtCP1 gradually increased, and the expression of NtCP2 first increased and then decreased, until it was lower than the unripe stage. Correlation analysis showed that the correlation between O^2-, H₂O₂and MDA content was high, and there was a significanly positive correlation between NtCP1 expression and the content of O^2-, H₂O₂and MDA, and a negative correlation between the expression of NtCP2 and the content of O^2-, H₂O₂and MDA. In conclusion, during the maturity of tobacco leaves, the active oxygen substances and MDA in the main vein were gradually accumulated, and the expressions of senescence related genes were also greatly affected. The research results provided a theoretical basis for the membrane lipid peroxidation in the main vein during the maturity of tobacco leaves.

Key words: flue-cured tobacco, main vein, maturity, reactive oxygen species, gene expression

范宁波1,周俊学2,江凯2,王宏2,史龙飞2,高玉龙3*,陈颐3*. 不同成熟期烤烟主脉中膜脂过氧化及其与衰老相关基因的关系探究[J]. 中国农业科技导报, 2021, 23(3): 66-72.

FAN Ningbo1, ZHOU Junxue2, JIANG Kai2, WANG Hong2, SHI Longfei2, GAO Yulong3*, CHEN Yi3*. Membrane Lipid Peroxidation and Its Relationship with Senescence-Related Genes in Main Veins of Flue-Cured Tobacco at Different Maturity Stages#br#[J]. Journal of Agricultural Science and Technology, 2021, 23(3): 66-72.

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不同成熟期烤烟主脉中膜脂过氧化及其与衰老相关基因的关系探究

Membrane Lipid Peroxidation and Its Relationship with Senescence-Related Genes in Main Veins of Flue-Cured Tobacco at Different Maturity Stages#br#

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