关键词: 板栗, 粉红单端孢菌, 病原菌鉴定, 生物学特性

Abstract: In order to clarify the pathogen and biological characteristics of chestnut pink disease, tissue isolation method was used to isolate and purify the pathogen; Koch′s rules, morphological characteristics and molecular biotechnology were used to identify the pathogen; and the effects of different temperature, humidity, medium, carbon and nitrogen sources, light and pH on the growth and sporulation of the pathogen were studied. On PDA medium,the mycelium of the pathogen was white at the initial stage of growth, while at the late stage,the central part of mycelium was orange pink, and the edge was light white with concentric annulation. The conidia were colorless and transparent, single cell, pear shape, smooth surface, with 0～1 diaphragma, without mediastinum, and constricted at the septum. The sequence similarity between its sequence of the pathogen and that of Trichoderma roseum (MN882763) was 100%. According to the morphological characteristics and molecular biotechnology, the pathogen of chestnut pink disease was Trichothecium roseum. The optimum growth conditions was gum acacia powder as carbon source, peptone as nitrogen source was, pH 8,temperature 25 ℃， PDA medium and 12 h light and dark alternate. The optimum conditions for sporulation was lactose as carbon source, peptone as nitrogen source, pH 7, temperature 25 ℃, beef extract peptone medium and 12 h light and dark alternate. Above results provided scientific basis for the control of the disease.

Key words: chestnut, Trichothecium roseum, pathogen identification, biological characteristics