三种镰刀菌引起的板栗内腐病病原菌鉴定

doi:10.13304/j.nykjdb.2021.0489

中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (7): 117-122.DOI: 10.13304/j.nykjdb.2021.0489

三种镰刀菌引起的板栗内腐病病原菌鉴定

张娜娜¹(), 温晓蕾¹^,²(), 李双民³, 冯丽娜¹, 霍佳欢¹, 兰淑慧¹, 栗佳宁¹, 郭思柔¹, 王建华¹, 齐慧霞¹()

^1.河北科技师范学院农学与生物科技学院，河北秦皇岛 066600
^2.河北农业大学植物保护学院，河北保定 071002
^3.河北省昌黎县职业技术教育中心，河北秦皇岛 066600

收稿日期:2021-06-15 接受日期:2022-05-22 出版日期:2022-07-15 发布日期:2022-08-15
通讯作者: 齐慧霞
作者简介:张娜娜 E-mail：924206346@qq.com
温晓蕾 E-mail：xiaoleiwen@sina.com第一联系人：张娜娜与温晓蕾为共同第一作者。
基金资助:
国家重点研发计划项目(2020YFD1000700);河北省教育厅平台项目（河北省板栗产业协同创新中心-2018-2023）

Identification of Pathogens of Three Kinds of Fusarium Causing Seed Rot of Chestnut

Nana ZHANG¹(), Xiaolei WEN¹^,²(), Shuangmin LI³, Lina FENG¹, Jiahuan HUO¹, Shuhui LAN¹, Jianing LI¹, Sirou GUO¹, Jianhua WANG¹, Huixia QI¹()

^1.College of Agronomy and Biotechnology，Hebei Normal University of Science＆Technology，Hebei qinhuangdao 066600，China
^2.College of Plant Protection，Hebei Agricultural University，Hebei Baoding 071001，China
^3.Vocational Education Center of Changli County，Hebei qinhuangdao 066600，China

Received:2021-06-15 Accepted:2022-05-22 Online:2022-07-15 Published:2022-08-15
Contact: Huixia QI

摘要/Abstract

摘要：

为明确板栗内腐病的病原，采用组织分离法分离纯化BL-5、BL-7和BL-9共3株病原菌，通过柯赫氏法则、形态学特征及分子生物学技术对病原菌进行鉴定。结果显示，BL-5在PDA培养基上初期菌丝白色，菌落中心部位有淡红色素，后期菌落逐渐变成深红色，菌落中心可见黄色菌丝；BL-7在PDA培养基上菌落呈白色、绒毛状，分生孢子长椭圆形或梨形、颜色透明、具隔膜；BL-9在PDA培养基上菌丝呈棉絮状，初期菌落呈粉红色，随着菌落的生长，后期菌落颜色逐渐变为浅黄色或褐色，分生孢子中间粗、两头较细长，似镰刀形，弯曲或直立，具有厚垣孢子。BL-5、BL-7、BL-9的ITS序列分别与Fusarium graminearum KJ847741、Fusarium proliferatum MW686898、Fusarium equiseti KU984711的序列同源性均达99%。因此，结合形态学特征和分子生物学特征，判断引起板栗内腐病的3种镰刀菌分别为禾谷镰刀菌（Fusarium graminearum）、层出镰孢菌（Fusarium proliferatum）、木贼镰刀菌（Fusarium equiseti），为板栗内腐病的有效防治提供了依据。

关键词: 板栗, 内腐病, 镰刀菌, 鉴定

Abstract:

To identify the pathogen of the chestnut endosepsis， 3 pathogens including BL-5， BL-7 and BL-9 were isolated and purified by tissue isolation method， and were identified by Koch’?s postulate， morphological characteristics and molecular biological techniques. The results showed that， in the initial stage， the hypha of BL-5 on PDA medium was white with light red pigment in the center of the colony， and the colony gradually turned dark red with yellow hypha observed in the center of the colony in the later stage. The colony of BL-7 on PDA medium was white and villous， and the conidia were oval or pear shaped with transparent color and septate. In addition， the hypha of BL-9 was cotton flocculent on PDA medium， the colony was pink in the initial stage. With the growth of the BL-9 colony， the color of the colony gradually changed to light yellow or brown， and the conidia was slender at both ends and thick in the middle， sickle like， curved or erect shape with chlamydospores. Furthermore， the ITS sequences of BL-5， BL-7 and BL-9 had 99% homology with Fusarium graminearum KJ847741， Fusarium proliferatum MW686898 and Fusarium equiseti KU984711， respectively. So， combining with morphological characteristics and molecular biology technology， BL-5， BL-7 and BL-9 were belonged to Fusarium graminearum， Fusarium proliferatum and Fusarium equiseti， respectively， which provided basis for the development of the effective pathogen control technology system.

Key words: chestnut, seed rot, Fusarium, identification

中图分类号:

S664.2

张娜娜, 温晓蕾, 李双民, 冯丽娜, 霍佳欢, 兰淑慧, 栗佳宁, 郭思柔, 王建华, 齐慧霞. 三种镰刀菌引起的板栗内腐病病原菌鉴定[J]. 中国农业科技导报, 2022, 24(7): 117-122.

Nana ZHANG, Xiaolei WEN, Shuangmin LI, Lina FENG, Jiahuan HUO, Shuhui LAN, Jianing LI, Sirou GUO, Jianhua WANG, Huixia QI. Identification of Pathogens of Three Kinds of Fusarium Causing Seed Rot of Chestnut[J]. Journal of Agricultural Science and Technology, 2022, 24(7): 117-122.

图/表 7

图1 BL-5的形态特征

Fig. 1 Morphological characteristics of the BL-5

图2 BL-7的形态特征

Fig. 2 Morphological characteristics of the BL-7

图3 BL-9的形态特征

Fig. 3 Morphological characteristics of the BL-9

图4 致病性测定

Fig. 4 Pathogenicity detection

图5 BL-5的系统发育树

Fig. 5 Phylogenetic tree of BL-5

图6 BL-7的系统发育树

Fig. 6 Phylogenetic tree of BL-7

图7 BL-9的系统发育树

Fig. 7 Phylogenetic tree of BL-9

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三种镰刀菌引起的板栗内腐病病原菌鉴定

Identification of Pathogens of Three Kinds of Fusarium Causing Seed Rot of Chestnut

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