枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理

doi:10.13304/j.nykjdb.2020.0991

中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (2): 152-159.DOI: 10.13304/j.nykjdb.2020.0991

枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理

梁艳琼(), 李锐, 吴伟怀, 习金根, 谭施北, 黄兴, 陆英, 贺春萍(), 易克贤()

中国热带农业科学院环境与植物保护研究所，农业农村部热带作物有害生物综合治理重点实验室，海南省热带农业有害生物检测监控重点实验室，海口 571101

收稿日期:2020-11-26 接受日期:2021-05-29 出版日期:2022-02-15 发布日期:2022-02-22
通讯作者: 贺春萍,易克贤
作者简介:梁艳琼 E-mail： yanqiongliang@126.com
基金资助:
海南省自然科学基金面上项目(320MS083);海南省科协青年科技英才学术创新计划项目(QCXM201714);国家天然橡胶产业技术体系建设项目(CARS-33-BC1);海南省院士创新平台科研专项

Inhibitory Mechanism of Volatilized Active Components from Bacillus subtilis Czk1 on Ganoderma pseudoferreum

Yanqiong LIANG(), Rui LI, Weihuai WU, Jingen XI, Shibei TAN, Xing HUANG, Ying LU, Chunping HE(), Kexian YI()

Key Laboratory of Integrated Pest Management on Tropical Crops，Ministry of Agriculture and Rural Affairs； Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests； Environment and Plant Protection Institute，Chinese Academy of Tropical Agricultural Sciences，Haikou 571101，China

Received:2020-11-26 Accepted:2021-05-29 Online:2022-02-15 Published:2022-02-22
Contact: Chunping HE,Kexian YI

摘要/Abstract

摘要：

红根病是我国分布广、发病率高、危害较为严重的橡胶树根部病害。为明确枯草芽孢杆菌Czk1菌株产生的挥发性物质对橡胶红根病原菌橡胶灵芝菌（Ganoderma pseudoferreum）的抑菌作用，以挥发性物质的主要成分苯甲醛、3，5-二甲氧基苯乙酮、2，6-二叔丁基对甲酚为研究对象，分析其对橡胶灵芝菌的糖、蛋白、DNA、关键代谢酶活性、细胞内容物泄露情况的影响。结果表明，经挥发物混合活性组分处理后，橡胶灵芝菌糖类含量显著降低；高浓度的枯草芽孢杆菌Czk1菌株挥发物混合活性组分影响其蛋白质的合成；DNA分子量变化不明显，未破坏橡胶灵芝菌DNA的一级结构。关键代谢酶活性（苹果酸脱氢酶、几丁质酶）降低，细胞内溶物泄漏情况明显。挥发物混合活性组分可能是通过破坏橡胶灵芝菌的细胞膜结构，扰乱物质代谢活动，从而达到抑菌作用。

关键词: 枯草芽孢杆菌, 橡胶灵芝菌, 挥发物活性组分, 抑菌机理

Abstract:

Red root disease is the root disease of rubber trees with wide distribution， high incidence， and more harmful in China. In order to determine the inhibitory effect of volatilized active components produced by Bacillus subtilis Czk1 strain on Ganoderma pseudoferreum， the dominant of active components， benzaldehyde， 3，5-dimethoxyacetophenone and butylated hydroxytoluene were used as research subjects to analyze their effects on sugar， protein， DNA， activity of key metabolic enzymes and cell content leakage of G. pseudoferreum. The results showed after G. pseudoferreum was treated with the active components of volatiled organic compounds， the sugar content of G. pseudoferreum decreased significantly， and the volatilized active components with high concentration affected the protein synthesis of G. pseudoferreum； the molecular weight of DNA did not change significantly， indicating the primary structure of DNA was not destoried； the activities of key metabolic enzymes （malate dehydrogenase and chitinase） decreased， and the leakage of intracellular lysates was obvious. The active components of volatile organic compounds achieved bacteriostasis by destroying the cell membrane structure and disrupting the metabolic activity of G. pseudoferreum.

Key words: Bacillus subtilis Czk1, Ganoderma pseudoferreum, volatilized active component, inhibitory mechanism

中图分类号:

S432

梁艳琼, 李锐, 吴伟怀, 习金根, 谭施北, 黄兴, 陆英, 贺春萍, 易克贤. 枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理[J]. 中国农业科技导报, 2022, 24(2): 152-159.

Yanqiong LIANG, Rui LI, Weihuai WU, Jingen XI, Shibei TAN, Xing HUANG, Ying LU, Chunping HE, Kexian YI. Inhibitory Mechanism of Volatilized Active Components from Bacillus subtilis Czk1 on Ganoderma pseudoferreum[J]. Journal of Agricultural Science and Technology, 2022, 24(2): 152-159.

图/表 6

图1 挥发物混合活性组分处理后橡胶灵芝菌细胞内容物泄露情况

Fig.1 Cellular content leakage of G. pseudoferreum after treated with volatilized active components

图2 挥发物混合活性组分处理后橡胶灵芝菌的糖含量注：图中不同字母表示在P<0.01水平差异显著。

Fig.2 Sugar content of G. pseudoferreum after treated with volatilized active componentsNote： Different letters in the figure indicate significant differences between different treatments at P <0.01 level.

图3 挥发物混合活性组分处理后橡胶灵芝菌蛋白含量变化注：图中不同字母表示在P<0.01水平差异显著。

Fig.3 Protein content of G.pseudoferreum after treated with volatilized active componentsNote： Different letters in the figure indicate significant differences different treatments at P <0.01 level.

图4 挥发物混合活性组分处理后橡胶灵芝菌苹果酸脱氢酶和几丁质酶的含量注：图中不同字母表示在P<0.01水平差异显著。

Fig.4 Activity of malate dehydrogenase and chitinase of G. pseudoferreum after treated with volatilized active componentsNote： Different letters in the figure indicate significant differences different treatments at P <0.01 level.

图5 挥发物混合活性组分处理后橡胶灵芝菌的丙二醛含量

Fig.5 Malondialdehyde content of G. pseudoferreum after treated with volatilized active components

图6 挥发物混合活性组分处理后橡胶灵芝菌的DNA注：M—DNA Marker 2000；1—空白对照；2—不加挥发物混合活性组分的病菌DNA；3—T1处理；4—T2处理。

Fig.6 DNA of G. pseudoferreum after treated with volatilized active componentsNote： M—DNA Marker 2000； 1—Control； 2—Not treated by volatilized active components； 3—T1 treatment； 4—T2 treatment.

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枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理

Inhibitory Mechanism of Volatilized Active Components from Bacillus subtilis Czk1 on Ganoderma pseudoferreum

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