大气压冷等离子体持续和间隔处理对燕麦种子萌发的影响

doi:10.13304/j.nykjdb.2022.0126

摘要/Abstract

摘要：

为了探究冷等离子体处理对燕麦种子萌发及幼苗生长的影响，用空气环境中大气压氩气交流放电冷等离子体射流对燕麦种子进行处理，对比分析了电压为4 kV、总处理时间分别为40和60 s时，持续处理和间隔处理对燕麦种子萌发及幼苗生长的影响。结果表明，与持续处理相比，间隔处理能够显著（P<0.05）提高种子的发芽势，其中10 s处理4次效果最佳，发芽势提高了约27.16%；间隔处理能够提高种子发芽率和幼苗的根长、芽长以及干物质积累，但是变化不显著。此外，间隔处理时燕麦种子的亲水性显著提高（P<0.05），10 s处理4次时亲水性变化最大，与持续处理相比增加约39.07%。相比于持续处理，间隔处理能够明显提高燕麦种子的发芽势、亲水性，为燕麦种植时缩短浸种时间、增加发芽整齐度提供理论指导。

关键词: 冷等离子体, 发芽率, 亲水性, α-淀粉酶活性

Abstract:

To study the effect of cold plasma treatment on oat seed germination and seedling growth， oat seeds were treated with a cold plasma jet at atmospheric pressure and argon AC discharge. The effects of continuous treatment and interval treatment on oat seed germination and seedling growth were compared and analyzed at a voltage of 4 kV and a total treatment time of 40 and 60 s， respectively. The results showed that interval treatment could significantly improve the germination potential of seeds compared with the continuous treatment （P<0.05）， and the 10 s treatment for 4 times had the greatest improvement， which was about 27.16% higher. Interval treatment could improve seed germination rate， root length， shoot length and dry matter accumulation of seedlings， but the change was not significant. Moreover， the hydrophilicity of oat seeds was significantly improved by interval treatment （P<0.05）， and the hydrophilicity changed the most when treated 4 times 10 s， which increased about 39.07% compared with continuous treatment. Compared with continuous treatment， interval treatment could significantly improve the germination potential， hydrophilicity and water uptake of oat seeds， which could provide a theoretical reference for shortening the soaking time of seeds and increasing the uniformity of germination when planting oats.

Key words: cold plasma, germination rate, hydrophilic, α-amylase activity

中图分类号:

S512.6

任棚, 吕晓桂, 石磊. 大气压冷等离子体持续和间隔处理对燕麦种子萌发的影响[J]. 中国农业科技导报, 2023, 25(7): 215-221.

Peng REN, Xiaogui LYU, Lei SHI. Effects of Atmospheric Pressure Cold Plasma Continuous and Interval Treatments on Oat Seed Germination[J]. Journal of Agricultural Science and Technology, 2023, 25(7): 215-221.

图/表 7

图1 等离子体射流装置注：Ar —氩气；1—高压交流电源；2—扁口状石英管；3—铝箔；4—等离子体射流；5—种子；6—传送带；7—高压探头；8—示波器；9—阴极。

Fig.1 Plasma jet deviceNote：Ar—Argon； 1—High voltage AC power supply； 2—Quartz tube； 3—Aluminum foil； 4—Plasma jet； 5—Seeds；6—Conveyor belt； 7—High voltage probe； 8—Oscilloscope； 9—Cathode.

表1 各组燕麦种子等离子体处理参数

Table 1 Plasma treatment parameters of each group oat seeds

组别

Group

处理时间

Treatment time/s

—

处理次数

Treatment times

—

图2 不同处理对燕麦种子亲水性和吸水率的影响注：不同小写字母表示处理之间在P<0.05水平差异显著。

Fig.2 Effects of different treatments on hydrophilicity and water uptake ratio of oat seedsNote：Different lowercase letters indicate significant differences between treatments at P<0.05 level.

图3 不同处理对燕麦种子发芽势和发芽率的影响注：不同小写字母表示处理之间在P<0.05水平差异显著。

Fig.3 Effects of different treatments on germination potential and rate of oat seedsNote：Different lowercase letters indicate significant differences between treatments at P<0.05 level.

图4 不同处理对燕麦幼苗生长的影响注：同一指标中不同小写字母表示处理之间在P<0.05水平差异显著。

Fig.4 Effects of different treatments on the growth of oat seedlingsNote：Different lowercase letters in same index indicate significant differences between treatments at P<0.05 level.

图5 不同处理对种子可溶性蛋白含量的影响注：不同小写字母表示处理之间在P<0.05水平差异显著。

Fig.5 Effects of different treatments on soluble protein content of seedsNote：Different lowercase letters indicate significant differences between treatments at P<0.05 level.

图6 不同处理对种子α-淀粉酶活性的影响注：不同小写字母表示处理之间在P<0.05水平差异显著。

Fig.6 Effects of different treatments on α-amylase activity of seedsNote：Different lowercase letters indicate significant differences between treatments at P<0.05 level.

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