油莎豆种质资源低温胁迫生理响应与耐寒性评价

doi:10.13304/j.nykjdb.2022.0132

中国农业科技导报 ›› 2023, Vol. 25 ›› Issue (9): 83-96.DOI: 10.13304/j.nykjdb.2022.0132

油莎豆种质资源低温胁迫生理响应与耐寒性评价

钟鹏¹(), 苗丽丽²(), 王建丽³, 刘杰², 王晓龙¹

^1.黑龙江省农业科学院畜牧兽医分院，黑龙江齐齐哈尔161005
^2.黑龙江省黑土保护利用研究院，哈尔滨150086
^3.黑龙江省农业科学院草业研究所，哈尔滨150086

收稿日期:2022-02-25 接受日期:2022-04-12 出版日期:2023-09-15 发布日期:2023-09-28
通讯作者: 钟鹏
作者简介:钟鹏E-mail：zhongpengvip@163.com
苗丽丽E-mail：miaolili2013@126.com第一联系人：钟鹏和苗丽丽为共同第一作者。
基金资助:
黑龙江省省属科研院所科研业务费项目(CZKYF2020C026);黑龙江省农业科学院“农业科技创新跨越工程”项目(2020KYGC0601);黑龙江省农业科学院成果转化中试项目(2020ZSXM002);黑龙江省农业科学院院级科研项目(2020YYYF012);黑龙江省农业科学院博士科研启动金项目

Physiological Response and Cold Resistance Evaluation of Cyperus esculentus Germplasms Under Low Temperature Stress

Peng ZHONG¹(), Lili MIAO²(), Jianli WANG³, Jie LIU², Xiaolong WANG¹

^1.Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Heilongjiang Qiqihar 161005, China
^2.Heilongjiang Academy of Black Soil Conservation and Utilization, Harbin 150086, China
^3.Institute of Pratacultural Science, Heilongjiang Academy of Agricultural Science, Harbin 150086, China

Received:2022-02-25 Accepted:2022-04-12 Online:2023-09-15 Published:2023-09-28
Contact: Peng ZHONG

摘要/Abstract

摘要：

低温是影响我国北方农牧交错区油莎豆种植的重要环境因子之一。为明确油莎豆耐低温主要鉴定指标并筛选耐低温较强的油莎豆种质资源，对不同生态区的18份油莎豆种质资源进行耐低温鉴定和评价。大田试验连续3年研究了倒春寒天气导致的低温胁迫对油莎豆出苗的影响，室内试验模拟倒春寒日最低温度范围设定了3、5、7、9 ℃共4个温度处理，测定低温处理后种子发芽相关指标和幼苗抗逆生理指标。结果表明，随着处理温度的逐渐降低，发芽指数、种子活力指数和叶片中叶绿素含量大致呈降低趋势，丙二醛不断积累，相对膜透性逐渐增大，可溶性糖、游离脯氨酸含量逐渐积累，超氧化物歧化酶和过氧化物酶活性总体上不断增强。通过大田试验、室内发芽试验及幼苗生理生化指标测定，综合评价将18份种质资源分为5组：HLJ105、RY126、BY156和HLJ106为强抗寒性种质材料，RY102和BY141为抗寒性材料，RY114、NM103、XJ101、JL154、LN181和SX100为中抗寒性材料，HB122和HN162为弱抗寒性材料，QY186、GD187、XY109和ZYS01为不抗寒性材料。研究结果为耐低温油莎豆种质资源创新和新品种培育提供了基础材料和理论依据。

关键词: 油莎豆, 倒春寒, 低温胁迫, 幼苗生理响应, 抗寒性评价

Abstract:

Low temperature is one of the important environmental factors affecting the cultivation of Cyperus esculentus in the agro-pastoral region of northern China. In order to clarify the main identification indexes and screen out the germplasm resources of Cyperus esculentus with strong low temperature tolerance， 18 Cyperus esculentus germplasms from different ecological regions were used to identify and evaluate their low temperature tolerance. The effect of low temperature stress on seedling emergence was studied in 3 consecutive years of late spring chilling. 3， 5， 7 and 9 ℃ were set to simulate the minimum temperature of late spring chilling， and seed germination related indexes and seedling stress resistance physiological indexes were measured to study the cold resistance. The results showed that with the decrease of temperature， germination index， seed vigor index and chlorophyll content in leaves decreased generally， relative membrane permeability increased， the contents of malonaldehyde， soluble sugar and free proline were gradually accumulated， and the activities of superoxide dismutase and peroxidase increased in the mass. Based on field test， laboratory germination test and physiological and biochemical indexes of seedlings， 18 germplasms were classified into 5 categories： strong cold resistance （HLJ105， RY126， BY156 and HLJ106）， cold resistance （RY102 and BY141）， moderate cold resistance （RY114， NM103， XJ101， JL154， LN181 and SX100）， weak cold resistance （HB122 and HN162）， and no cold resistance （QY186， GD187， XY109 and ZYS01）. The results provided basic resources and theoretical basis for the germplasms innovation and cultivars breeding of low temperature resistant Cyperus esculentus.

Key words: Cyperus esculentus, late spring chilling, low temperature stress, seedling physiological responses, cold resistance evaluation

中图分类号:

S565

钟鹏, 苗丽丽, 王建丽, 刘杰, 王晓龙. 油莎豆种质资源低温胁迫生理响应与耐寒性评价[J]. 中国农业科技导报, 2023, 25(9): 83-96.

Peng ZHONG, Lili MIAO, Jianli WANG, Jie LIU, Xiaolong WANG. Physiological Response and Cold Resistance Evaluation of Cyperus esculentus Germplasms Under Low Temperature Stress[J]. Journal of Agricultural Science and Technology, 2023, 25(9): 83-96.

图/表 12

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种质材料 Germplasm	种质来源 Origin	出苗率 Seedling emergence rate/%
种质材料 Germplasm	种质来源 Origin	2018	2019	2020
HLJ105	黑龙江Heilongjiang	95.18±1.33 a	96.94±2.26 a	93.52±3.34 a
RY126	俄罗斯Russia	93.22±1.68 a	97.82±1.57 a	92.84±3.18 a
BY156	保加利亚Bulgaria	93.14±1.52 a	95.73±2.04 a	91.08±1.69 ab
HLJ106	黑龙江Heilongjiang	90.16±1.69 a	92.48±3.38 a	88.87±4.52 ab
RY102	俄罗斯Russia	89.44±1.87 a	91.92±2.11 b	85.52±5.64 abc
BY141	保加利亚Bulgaria	88.02±2.26 ab	86.61±3.18 bcd	83.16±1.87 bc
RY114	俄罗斯Russia	86.69±2.84 bc	85.16±4.97 bcd	82.15±6.62 cd
NM103	内蒙古Inner Mongolia	83.17±1.89 bcd	83.94±1.49 cd	85.91±3.58 abc
XJ101	新疆Xinjiang	80.35±3.34 cd	78.82±3.37 de	86.13±2.49 abc
JL154	吉林Jilin	80.11±2.66 cd	82.25±4.42 cde	72.15±5.47 d
LN181	辽宁Liaoning	78.10±2.15 de	75.48±3.55 ef	70.38±6.11 d
SX100	山西Shanxi	72.64±1.51 e	70.63±4.77 f	73.64±3.42 d
HB122	河北Hebei	65.54±4.43 f	66.12±2.58 f	62.04±4.97 e
HN162	河南Henan	53.13±3.37 g	55.47±6.83 g	50.35±7.74 f
ZYS01	湖北Hubei	45.87±5.52 hi	48.96±7.24 h	41.18±7.26 g
XY109	湖南Hunan	44.69±3.96 i	43.32±6.08 h	43.51±6.83 g
GD187	广东Guangdong	42.35±6.65 i	43.17±5.56 h	40.12±5.44 g
QY186	海南Hainan	40.06±7.84 i	41.66±6.84 h	38.68±8.85 g

种质材料 Germplasm	种质来源 Origin	出苗率 Seedling emergence rate/%
种质材料 Germplasm	种质来源 Origin	2018	2019	2020
HLJ105	黑龙江Heilongjiang	95.18±1.33 a	96.94±2.26 a	93.52±3.34 a
RY126	俄罗斯Russia	93.22±1.68 a	97.82±1.57 a	92.84±3.18 a
BY156	保加利亚Bulgaria	93.14±1.52 a	95.73±2.04 a	91.08±1.69 ab
HLJ106	黑龙江Heilongjiang	90.16±1.69 a	92.48±3.38 a	88.87±4.52 ab
RY102	俄罗斯Russia	89.44±1.87 a	91.92±2.11 b	85.52±5.64 abc
BY141	保加利亚Bulgaria	88.02±2.26 ab	86.61±3.18 bcd	83.16±1.87 bc
RY114	俄罗斯Russia	86.69±2.84 bc	85.16±4.97 bcd	82.15±6.62 cd
NM103	内蒙古Inner Mongolia	83.17±1.89 bcd	83.94±1.49 cd	85.91±3.58 abc
XJ101	新疆Xinjiang	80.35±3.34 cd	78.82±3.37 de	86.13±2.49 abc
JL154	吉林Jilin	80.11±2.66 cd	82.25±4.42 cde	72.15±5.47 d
LN181	辽宁Liaoning	78.10±2.15 de	75.48±3.55 ef	70.38±6.11 d
SX100	山西Shanxi	72.64±1.51 e	70.63±4.77 f	73.64±3.42 d
HB122	河北Hebei	65.54±4.43 f	66.12±2.58 f	62.04±4.97 e
HN162	河南Henan	53.13±3.37 g	55.47±6.83 g	50.35±7.74 f
ZYS01	湖北Hubei	45.87±5.52 hi	48.96±7.24 h	41.18±7.26 g
XY109	湖南Hunan	44.69±3.96 i	43.32±6.08 h	43.51±6.83 g
GD187	广东Guangdong	42.35±6.65 i	43.17±5.56 h	40.12±5.44 g
QY186	海南Hainan	40.06±7.84 i	41.66±6.84 h	38.68±8.85 g

种质材料 Germplasm	对照 Control	处理Treatment
种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
HLJ105	21.45±1.68 ab	13.16±1.22 a	15.54±1.42 a	18.66±1.68 a	20.87±1.62 ab
RY126	22.08±2.32 ab	12.58±1.34 a	14.87±1.53 a	18.24±1.62 ab	21.56±1.77 a
BY156	21.14±1.54 ab	12.44±1.06 a	14.82±1.44 a	17.92±1.54 ab	20.69±1.39 ab
HLJ106	22.96±1.86 a	13.81±1.55 a	15.73±1.38 a	18.79±1.39 a	21.84±1.84 a
RY102	20.13±3.31 ab	11.01±1.18 b	13.20±1.32 b	17.55±1.66 ab	20.01±1.58 ab
BY141	21.54±3.27 ab	10.25±1.11 bc	13.06±1.26 b	17.23±1.52 ab	20.78±1.46 ab
RY114	21.67±2.52 ab	10.54±1.26 bc	12.11±1.24 bc	15.97±1.46 c	21.23±1.72 a
NM103	22.55±1.98 a	11.36±1.07 b	12.08±1.30 bc	17.59±1.37 ab	21.97±1.35 a
XJ101	19.94±2.11 ab	9.54±1.02 c	11.54±1.29 bcd	16.32±1.25 c	19.64±1.26 b
JL154	20.86±1.60 ab	9.68±1.02 c	10.67±1.17 de	17.69±1.44 ab	20.25±1.80 ab
LN181	20.53±2.73 ab	10.23±1.13 bc	11.34±1.43 cd	16.67±1.22 bc	20.14±1.74 ab
SX100	19.81±2.47 ab	9.94±1.07 bc	10.25±1.38 de	15.98±1.34 c	19.63±1.69 b
HB122	20.53±1.65 ab	10.23±1.15 bc	11.09±1.21cd	16.52±1.09 c	19.98±1.65 b
HN162	21.25±2.20 ab	9.06±1.14 cd	10.52±1.15 de	17.34±1.17 ab	20.85±1.52 ab
ZYS01	22.30±3.37 ab	8.87±1.09 d	9.66±1.07 e	15.50±1.58 c	21.29±1.38 a
XY109	19.11±3.05 b	7.65±1.00 d	9.51±1.18 e	16.23±1.33 c	19.08±1.22 b
GD187	19.62±1.88 ab	8.22±1.01 d	9.90±1.02 e	16.08±1.19 c	19.24±1.66 b
QY186	21.87±2.04 ab	7.54±1.05 d	9.29±1.11 e	15.91±1.84 c	21.10±1.51 a

种质材料 Germplasm	对照 Control	处理Treatment
种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
HLJ105	21.45±1.68 ab	13.16±1.22 a	15.54±1.42 a	18.66±1.68 a	20.87±1.62 ab
RY126	22.08±2.32 ab	12.58±1.34 a	14.87±1.53 a	18.24±1.62 ab	21.56±1.77 a
BY156	21.14±1.54 ab	12.44±1.06 a	14.82±1.44 a	17.92±1.54 ab	20.69±1.39 ab
HLJ106	22.96±1.86 a	13.81±1.55 a	15.73±1.38 a	18.79±1.39 a	21.84±1.84 a
RY102	20.13±3.31 ab	11.01±1.18 b	13.20±1.32 b	17.55±1.66 ab	20.01±1.58 ab
BY141	21.54±3.27 ab	10.25±1.11 bc	13.06±1.26 b	17.23±1.52 ab	20.78±1.46 ab
RY114	21.67±2.52 ab	10.54±1.26 bc	12.11±1.24 bc	15.97±1.46 c	21.23±1.72 a
NM103	22.55±1.98 a	11.36±1.07 b	12.08±1.30 bc	17.59±1.37 ab	21.97±1.35 a
XJ101	19.94±2.11 ab	9.54±1.02 c	11.54±1.29 bcd	16.32±1.25 c	19.64±1.26 b
JL154	20.86±1.60 ab	9.68±1.02 c	10.67±1.17 de	17.69±1.44 ab	20.25±1.80 ab
LN181	20.53±2.73 ab	10.23±1.13 bc	11.34±1.43 cd	16.67±1.22 bc	20.14±1.74 ab
SX100	19.81±2.47 ab	9.94±1.07 bc	10.25±1.38 de	15.98±1.34 c	19.63±1.69 b
HB122	20.53±1.65 ab	10.23±1.15 bc	11.09±1.21cd	16.52±1.09 c	19.98±1.65 b
HN162	21.25±2.20 ab	9.06±1.14 cd	10.52±1.15 de	17.34±1.17 ab	20.85±1.52 ab
ZYS01	22.30±3.37 ab	8.87±1.09 d	9.66±1.07 e	15.50±1.58 c	21.29±1.38 a
XY109	19.11±3.05 b	7.65±1.00 d	9.51±1.18 e	16.23±1.33 c	19.08±1.22 b
GD187	19.62±1.88 ab	8.22±1.01 d	9.90±1.02 e	16.08±1.19 c	19.24±1.66 b
QY186	21.87±2.04 ab	7.54±1.05 d	9.29±1.11 e	15.91±1.84 c	21.10±1.51 a

种质材料 Germplasm	对照 Control	处理Treatment
种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
HLJ105	116.42±10.24 a	72.16±5.87 a	85.47±5.45 a	105.81±8.24 a	114.19±8.24 a
RY126	115.37±9.68 ab	73.54±4.62 a	84.12±6.17 a	106.11±9.12 a	111.58±9.92 a
BY156	106.83±9.36 ab	70.08±5.42 a	84.87±5.54 a	101.54±7.37 ab	103.92±6.88 ab
HLJ106	109.58±8.47 ab	71.14±6.03 a	83.95±6.35 a	104.32±6.24 a	106.55±10.37 ab
RY102	112.46±6.69 ab	63.44±5.29 b	83.01±5.81 a	103.36±7.08 ab	109.87±9.75 ab
BY141	102.17±10.55 b	60.12±4.67 b	82.16±4.68 a	98.45±6.51 abc	99.82±5.38 c
RY114	98.84±11.64 b	62.94±4.33 b	73.66±5.22 b	91.24±6.33 c	95.46±6.51 c
NM103	100.25±10.23 b	58.93±3.69 bc	70.18±4.91 bc	96.52±4.48 c	96.17±5.84 c
XJ101	97.89±8.64 b	61.88±5.22 b	72.64±6.14 bc	89.95±5.29 c	93.61±8.26 c
JL154	98.15±8.87 b	56.81±4.14 cd	70.88±6.03 bc	91.23±6.17 c	93.54±7.95 c
LN181	114.37±9.22 ab	54.27±4.61 cd	69.87±5.29 bc	102.39±4.62 ab	108.57±8.83 ab
SX100	104.28±10.05 b	52.39±3.85 d	71.03±5.07 bc	94.67±5.83 c	100.63±9.07 bc
HB122	106.42±9.06 ab	53.97±4.07 c	68.24±6.23 bc	96.58±6.19 b	104.87±8.15 ab
HN162	101.09±9.94 b	57.04±3.15 cd	66.57±5.16 c	94.66±4.55 c	97.85±4.58 c
ZYS01	111.04±8.98 ab	45.51±4.10 e	60.15±4.54 d	99.47±3.87 abc	104.52±5.09 ab
XY109	98.83±10.51 b	43.67±3.94 e	58.07±4.68 d	93.53±5.46 c	97.19±8.66 c
GD187	85.47±10.24 c	42.55±3.16 e	56.69±5.00 d	74.56±6.86 d	83.14±6.10 d
QY186	95.33±9.76 b	43.90±2.68 e	57.32±4.27 d	91.12±5.04 c	94.07±5.94 c

油莎豆种质资源低温胁迫生理响应与耐寒性评价

Physiological Response and Cold Resistance Evaluation of Cyperus esculentus Germplasms Under Low Temperature Stress

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Metrics

生理指标 Physical index	种质材料 Germplasm	对照 Control	处理Treatment
生理指标 Physical index	种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
相对膜透性 Relative membrane permeability/%	HLJ105	15.39±1.34 ab	25.52±1.87 e	23.11±1.55 e	19.24±1.47 d	17.16±1.61 b
	RY126	16.21±1.67 a	26.37±1.69 e	23.67±1.62 e	18.87±1.49 d	18.04±1.57 ab
	BY156	15.48±1.14 ab	25.41±1.54 e	22.54±1.48 e	19.23±1.52 d	17.03±1.59 b
	HLJ106	16.37±1.52 a	25.83±1.77 e	23.39±1.50 e	19.55±1.36 d	17.95±1.64 ab
	RY102	15.24±1.84 ab	29.08±1.63 d	26.96±1.73 cd	20.14±1.22 cd	17.37±1.43 b
	BY141	15.88±1.65 ab	31.33±1.84 bc	27.02±1.64 cd	19.96±1.31 cd	17.62±1.35 b
	RY114	16.32±1.53 a	30.56±1.95 cd	28.87±1.29 bc	20.08±1.40 cd	18.16±1.61 ab
	NM103	15.75±1.16 ab	31.29±1.87 bc	29.24±1.63 b	20.41±1.29 cd	18.11±1.55 ab
	XJ101	15.49±1.27 ab	31.54±1.80 bc	29.16±1.71 bc	19.57±1.38 d	17.29±1.38 b
	JL154	16.39±1.39 a	32.59±1.53 b	29.41±1.86 b	21.33±1.15 abc	18.17±1.67 ab
	LN181	15.98±1.28 ab	31.64±1.71 bc	28.43±1.80 bc	20.34±1.33 cd	18.21±1.83 ab
	SX100	14.26±1.05 b	29.22±1.94 cd	26.44±1.47 d	20.58±1.23 bc	18.98±1.39 ab
	HB122	16.81±1.63 a	31.58±1.85 bc	27.19±1.69 cd	20.61±1.54 bc	18.95±1.50 ab
	HN162	15.54±1.97 ab	31.02±1.99 bcd	27.24±1.22 cd	21.35±1.58 ab	19.14±1.22 ab
	ZYS01	14.86±1.21 b	34.83±2.04 a	31.52±1.93 a	22.46±1.46 a	18.99±1.71 ab
	XY109	16.02±1.32 ab	35.57±2.13 a	32.25±1.95 a	21.93±1.50 ab	19.33±1.29 ab
	GD187	15.20±1.08 ab	35.06±1.97 a	31.94±1.75 a	21.84±1.37 ab	19.65±1.84 a
	QY186	15.14±1.57 ab	36.18±2.15 a	32.68±1.82 a	22.06±1.55 ab	19.50±1.67 a
丙二醛含量 Malondialdehyde content/ （μmol·g^-1FW）	HLJ105	7.26±0.61 a	12.56±1.12 c	10.43±1.09 c	9.32±1.02 c	8.14±0.54 c
	RY126	7.14±0.84 a	13.14±1.24 c	11.12±1.14 c	9.41±1.14 c	8.02±0.62 c
	BY156	7.38±0.73 a	12.73±1.32 c	10.52±1.12 c	9.24±1.08 c	8.15±0.67 c
	HLJ106	7.43±0.62 a	13.56±1.27 c	10.81±1.03 c	9.35±1.04 c	8.06±0.53 c
	RY102	7.21±0.53 a	16.44±1.52 b	13.66±1.27 b	10.26±1.15 ab	8.25±0.48 c
	BY141	7.05±0.49 a	17.35±1.58 b	14.15±1.36 b	10.64±1.11 ab	8.34±0.57 abc
	RY114	6.94±0.50 a	17.19±1.60 b	13.94±1.30 b	9.58±1.02 c	8.22±0.66 c
	NM103	7.13±0.51 a	16.87±1.53 b	13.27±1.29 b	10.81±1.33 ab	8.78±0.65 abc
	XJ101	7.08±0.57 a	16.92±1.42 b	13.40±1.44 b	10.23±0.98 ab	8.44±0.87 abc
	JL154	6.88±0.66 a	17.66±1.38 b	14.25±1.28 b	10.19±1.08 ab	8.36±0.46 abc
	LN181	7.33±0.75 a	17.80±1.54 b	14.17±1.36 b	10.55±0.94 ab	8.19±0.37 c
	SX100	6.75±0.73 a	17.43±1.44 b	13.94±1.42 b	9.94±1.16 c	8.27±0.49 bc
	HB122	7.25±0.82 a	18.03±1.59 b	14.65±1.38 b	10.37±0.92 ab	8.43±0.70 abc
	HN162	6.78±0.61 a	18.17±1.61 b	14.71±1.51 b	10.02±0.89 bc	8.82±0.68 abc
	ZYS01	7.10±0.53 a	20.26±1.68 a	17.84±1.50 a	11.35±1.14 ab	8.94±0.73 ab
	XY109	7.24±0.44 a	20.14±1.53 a	17.55±1.49 a	11.26±1.21 ab	9.13±0.82 ab
	GD187	6.93±0.81 a	19.97±1.72 a	17.17±1.52 a	11.48±1.05 a	9.08±0.65 ab
	QY186	7.36±0.76 a	20.35±1.76 a	17.93±1.47 a	11.50±1.11 a	9.16±0.77 a

材料 Material	隶属函数值 Subordinate function value											抗寒性综合评价值 Cold-resistance integrated assessment value	抗寒力评价Evaluation of cold resistance ability
材料 Material	出苗率ER	出苗时间ED	发芽指数GI	种子活力VI	叶绿素 Chl（a+b）	相对膜透性RMP	丙二醛 MDA	游离脯氨酸Pro	可溶性糖SS	超氧化物歧化酶SOD	过氧化物酶POD	抗寒性综合评价值 Cold-resistance integrated assessment value	抗寒力评价Evaluation of cold resistance ability
HLJ105	0.819 9	0.761 7	0.613 5	0.819 8	0.768 2	0.703 1	0.778 0	0.757 8	0.552 7	0.604 2	0.615 9	0.708 6	Ⅰ
RY126	0.814 1	0.757 0	0.569 7	0.837 4	0.792 5	0.714 7	0.743 4	0.780 0	0.550 6	0.608 4	0.669 0	0.712 4	Ⅰ
BY156	0.802 7	0.746 5	0.588 5	0.856 0	0.743 1	0.709 2	0.779 7	0.805 4	0.529 2	0.621 2	0.687 1	0.715 3	Ⅰ
HLJ106	0.779 7	0.724 0	0.601 5	0.849 2	0.762 3	0.733 8	0.747 9	0.743 1	0.577 5	0.605 4	0.635 5	0.705 4	Ⅰ
RY102	0.762 5	0.711 7	0.546 9	0.664 1	0.697 1	0.624 1	0.638 6	0.600 1	0.458 4	0.513 4	0.559 4	0.616 0	Ⅱ
BY141	0.733 9	0.687 4	0.475 9	0.688 4	0.614 6	0.606 9	0.606 3	0.627 6	0.487 5	0.509 8	0.578 4	0.601 5	Ⅱ
RY114	0.722 4	0.6773	0.486 4	0.736 8	0.613 7	0.534 0	0.503 7	0.609 6	0.446 8	0.422 4	0.521 6	0.570 4	Ⅲ
NM103	0.616 7	0.674 7	0.503 8	0.687 8	0.514 1	0.403 4	0.522 6	0.580 0	0.439 6	0.559 4	0.578 1	0.552 7	Ⅲ
XJ101	0.628 1	0.554 1	0.478 4	0.732 1	0.468 5	0.391 1	0.518 4	0.561 7	0.511 1	0.306 4	0.608 8	0.523 5	Ⅲ
JL154	0.670 8	0.525 4	0.464 0	0.678 8	0.359 7	0.402 9	0.389 6	0.415 5	0.449 4	0.330 4	0.465 7	0.468 4	Ⅲ
LN181	0.542 1	0.497 2	0.498 3	0.574 5	0.377 8	0.305 1	0.311 8	0.315 1	0.406 3	0.227 4	0.486 8	0.412 9	Ⅲ
SX100	0.513 5	0.478 4	0.501 8	0.402 4	0.386 5	0.388 0	0.387 3	0.324 2	0.420 6	0.248 9	0.435 5	0.407 9	Ⅲ
HB122	0.561 9	0.316 5	0.498 3	0.407 1	0.306 5	0.332 3	0.302 1	0.389 6	0.435 5	0.231 0	0.307 3	0.371 6	Ⅳ
HN162	0.452 9	0.323 9	0.426 4	0.464 2	0.302 7	0.301 0	0.273 1	0.317 1	0.464 9	0.229 3	0.343 5	0.354 5	Ⅳ
ZYS01	0.389 9	0.262 7	0.397 8	0.309 9	0.276 7	0.226 6	0.250 4	0.209 1	0.254 7	0.176 1	0.170 1	0.265 8	Ⅴ
XY109	0.378 4	0.250 7	0.300 3	0.341 9	0.209 4	0.250 4	0.259 5	0.205 1	0.309 5	0.187 7	0.192 3	0.262 3	Ⅴ
GD187	0.361 2	0.235 0	0.319 0	0.397 8	0.216 0	0.233 5	0.247 0	0.242 2	0.259 1	0.151 4	0.143 9	0.255 1	Ⅴ
QY186	0.344 0	0.221 1	0.244 8	0.360 5	0.295 8	0.218 5	0.261 7	0.201 9	0.279 9	0.173 5	0.180 3	0.252 9	Ⅴ

指标 Index	抗寒性综合评价值IAV	出苗率 ER	出苗时间 ED	发芽指数 GI	种子活力 VI	叶绿素 Chl（a+b）	相对膜透性RMP	丙二醛 MDA	游离脯氨酸Pro	可溶性糖 SS	超氧化物歧化酶SOD
出苗率 ER	0.978 2^*
出苗时间 ED	-0.912 8^*	-0.921 8^*
发芽指数 GI	0.953 6^*	0.996 5^**	-0.981 6^*
种子活力 VI	0.814 5	0.989 4^**	-0.980 2^*	0.998 6^**
叶绿素 Chl（a+b）	0.762 4	0.701 6	-0.650 7	0.812 7	0.713 8
相对膜透性 RMP	-0.754 9	-0.834 7	0.972 5^*	-0.796 3	-0.698 7	-0.984 6^*
丙二醛 MDA	-0.923 3^*	-0.874 2	0.971 6^*	-0.869 7	-0.761 5	-0.965 1^*	0.982 6^*
游离脯氨酸 Pro	0.836 9	0.731 5	-0.684 3	0.874 2	0.753 9	0.894 3	-0.869 1	-0.894 7
可溶性糖 SS	0.684 2	0.493 8	-0.521 7	0.654 4	0.539 7	0.648 8	-0.564 3	-0.468 3	0.823 9
超氧化物歧化酶SOD	0.970 1^*	0.961 8^*	-0.714 9	0.821 9	0.714 5	0.972 5^*	-0.762 5	-0.976 8^*	0.781 3	0.584 9
过氧化物酶 POD	0.614 5	0.574 3	-0.486 1	0.668 5	0.582 2	0.521 7	-0.354 9	-0.762 9	0.986 5^*	0.812 6	0.794 1

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生理指标 Physical index	种质材料 Germplasm	对照 Control	处理Treatment
生理指标 Physical index	种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
游离脯氨酸含量 Free proline content/ （μmol·g^-1FW）	HLJ105	60.43±4.36 a	136.67±6.82 a	115.26±7.19 a	96.87±7.51 a	80.26±6.39 a
	RY126	58.55±5.87 a	139.42±7.49 a	109.84±7.38 ab	93.64±6.39 a	78.34±5.47 ab
	BY156	56.37±6.23 a	142.87±5.68 a	110.63±6.55 ab	95.58±7.25 a	79.52±6.11 a
	HLJ106	61.94±4.22 a	135.56±7.44 a	108.49±4.31 ab	92.15±5.37 a	73.19±6.37 abc
	RY102	60.52±5.01 a	121.06±8.62 b	106.64±5.57 ab	95.34±6.64 a	71.44±5.98 bcd
	BY141	56.54±6.44 a	119.68±6.37 b	104.86±8.06 b	96.62±7.66 a	76.46±7.36 abc
	RY114	59.00±6.89 a	120.31±8.82 b	91.25±7.94 c	91.37±4.23 a	75.91±7.81 abc
	NM103	60.57±5.21 a	116.47±7.39 b	95.64±6.82 c	81.21±7.58 b	77.08±6.43 ab
	XJ101	61.11±4.66 a	113.53±7.24 b	96.37±5.94 c	79.35±6.94 b	78.49±5.17 ab
	JL154	58.96±5.79 a	121.68±8.67 b	93.14±6.73 cd	76.22±6.08 bc	72.66±6.56 bcd
	LN181	57.34±6.04 a	118.25±8.90 b	90.18±5.86 cd	71.84±7.13 cd	70.28±4.98 bcd
	SX100	56.08±5.91 a	117.86±6.33 b	86.64±6.21 de	70.69±4.27 cd	70.19±6.73 bcd
	HB122	61.22±7.36 a	119.95±5.21 b	89.97±4.82 cd	71.38±3.64 cd	69.92±5.14 cd
	HN162	58.23±3.48 a	120.58±6.78 b	81.65±3.67 e	70.11±4.65 cd	70.03±6.16 bcd
	ZYS01	59.37±6.62 a	100.47±8.29 c	80.23±4.88 e	72.15±4.77 cd	72.10±5.99 bcd
	XY109	61.58±7.13 a	103.52±7.55 c	81.36±6.98 e	68.86±6.62 d	67.89±4.88 cd
	GD187	56.54±5.46 a	101.37±6.94 c	79.84±7.06 ef	70.39±5.97 cd	69.96±6.05 cd
	QY186	59.92±5.75 a	100.18±6.32 c	73.67±6.11 f	67.83±6.14 d	66.83±5.41 d
可溶性糖含量 Soluble sugar/（mg·g^-1FW）	HLJ105	7.25±1.06 a	16.21±1.36 a	13.68±1.22 a	9.89±1.05 a	8.94±0.65 a
	RY126	7.68±1.12 a	17.09±1.55 a	13.54±1.03 a	9.24±1.13 a	8.65±0.72 ab
	BY156	8.06±0.98 a	17.12±1.21 a	12.49±0.84 ab	9.36±0.84 a	8.96±0.87 a
	HLJ106	7.11±1.37 a	16.83±0.98 a	13.17±1.09 ab	9.08±0.62 ab	8.63±0.64 ab
	RY102	8.14±1.45 a	15.03±1.05 b	12.55±0.86 ab	9.17±1.17 ab	9.11±0.88 a
	BY141	7.36±0.84 a	14.36±0.88 bc	12.38±0.91 ab	9.28±0.93 a	8.82±0.51 ab
	RY114	7.85±1.12 a	14.19±1.17 bc	12.13±1.14 b	8.76±0.76 ab	8.59±0.92 ab
	NM103	7.66±1.23 a	13.67±1.31 bc	10.15±1.03 cd	8.13±0.81 ab	8.07±0.37 bc
	XJ101	7.25±0.99 a	14.83±1.02 bc	10.67±0.75 c	8.05±1.09 c	8.01±0.46 bc
	JL154	7.46±1.07 a	13.55±0.74 bc	10.02±0.61 cd	8.12±0.68 ab	8.04±0.68 bc
	LN181	7.89±0.72 a	13.29±0.95 c	9.64±1.08 cde	8.24±0.52 ab	8.16±0.73 bc
	SX100	8.14±0.54 a	14.05±1.04 bc	10.33±0.79 cd	8.52±1.04 ab	8.23±0.59 abc
	HB122	8.05±1.29 a	14.26±1.28 bc	10.25±1.01 cd	8.49±0.96 ab	8.12±0.76 bc
	HN162	7.48±1.24 a	13.98±0.41 bc	9.68±0.65 cde	7.87±0.43 c	7.96±0.44 bc
	ZYS01	7.61±0.78 a	10.21±1.22 d	9.24±0.47 de	8.04±0.66 c	7.91±0.82 c

生理指标 Physical index	种质材料 Germplasm	对照 Control	处理Treatment
生理指标 Physical index	种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
游离脯氨酸含量 Free proline content/ （μmol·g^-1FW）	HLJ105	60.43±4.36 a	136.67±6.82 a	115.26±7.19 a	96.87±7.51 a	80.26±6.39 a
	RY126	58.55±5.87 a	139.42±7.49 a	109.84±7.38 ab	93.64±6.39 a	78.34±5.47 ab
	BY156	56.37±6.23 a	142.87±5.68 a	110.63±6.55 ab	95.58±7.25 a	79.52±6.11 a
	HLJ106	61.94±4.22 a	135.56±7.44 a	108.49±4.31 ab	92.15±5.37 a	73.19±6.37 abc
	RY102	60.52±5.01 a	121.06±8.62 b	106.64±5.57 ab	95.34±6.64 a	71.44±5.98 bcd
	BY141	56.54±6.44 a	119.68±6.37 b	104.86±8.06 b	96.62±7.66 a	76.46±7.36 abc
	RY114	59.00±6.89 a	120.31±8.82 b	91.25±7.94 c	91.37±4.23 a	75.91±7.81 abc
	NM103	60.57±5.21 a	116.47±7.39 b	95.64±6.82 c	81.21±7.58 b	77.08±6.43 ab
	XJ101	61.11±4.66 a	113.53±7.24 b	96.37±5.94 c	79.35±6.94 b	78.49±5.17 ab
	JL154	58.96±5.79 a	121.68±8.67 b	93.14±6.73 cd	76.22±6.08 bc	72.66±6.56 bcd
	LN181	57.34±6.04 a	118.25±8.90 b	90.18±5.86 cd	71.84±7.13 cd	70.28±4.98 bcd
	SX100	56.08±5.91 a	117.86±6.33 b	86.64±6.21 de	70.69±4.27 cd	70.19±6.73 bcd
	HB122	61.22±7.36 a	119.95±5.21 b	89.97±4.82 cd	71.38±3.64 cd	69.92±5.14 cd
	HN162	58.23±3.48 a	120.58±6.78 b	81.65±3.67 e	70.11±4.65 cd	70.03±6.16 bcd
	ZYS01	59.37±6.62 a	100.47±8.29 c	80.23±4.88 e	72.15±4.77 cd	72.10±5.99 bcd
	XY109	61.58±7.13 a	103.52±7.55 c	81.36±6.98 e	68.86±6.62 d	67.89±4.88 cd
	GD187	56.54±5.46 a	101.37±6.94 c	79.84±7.06 ef	70.39±5.97 cd	69.96±6.05 cd
	QY186	59.92±5.75 a	100.18±6.32 c	73.67±6.11 f	67.83±6.14 d	66.83±5.41 d
可溶性糖含量 Soluble sugar/（mg·g^-1FW）	HLJ105	7.25±1.06 a	16.21±1.36 a	13.68±1.22 a	9.89±1.05 a	8.94±0.65 a
	RY126	7.68±1.12 a	17.09±1.55 a	13.54±1.03 a	9.24±1.13 a	8.65±0.72 ab
	BY156	8.06±0.98 a	17.12±1.21 a	12.49±0.84 ab	9.36±0.84 a	8.96±0.87 a
	HLJ106	7.11±1.37 a	16.83±0.98 a	13.17±1.09 ab	9.08±0.62 ab	8.63±0.64 ab
	RY102	8.14±1.45 a	15.03±1.05 b	12.55±0.86 ab	9.17±1.17 ab	9.11±0.88 a
	BY141	7.36±0.84 a	14.36±0.88 bc	12.38±0.91 ab	9.28±0.93 a	8.82±0.51 ab
	RY114	7.85±1.12 a	14.19±1.17 bc	12.13±1.14 b	8.76±0.76 ab	8.59±0.92 ab
	NM103	7.66±1.23 a	13.67±1.31 bc	10.15±1.03 cd	8.13±0.81 ab	8.07±0.37 bc
	XJ101	7.25±0.99 a	14.83±1.02 bc	10.67±0.75 c	8.05±1.09 c	8.01±0.46 bc
	JL154	7.46±1.07 a	13.55±0.74 bc	10.02±0.61 cd	8.12±0.68 ab	8.04±0.68 bc
	LN181	7.89±0.72 a	13.29±0.95 c	9.64±1.08 cde	8.24±0.52 ab	8.16±0.73 bc
	SX100	8.14±0.54 a	14.05±1.04 bc	10.33±0.79 cd	8.52±1.04 ab	8.23±0.59 abc
	HB122	8.05±1.29 a	14.26±1.28 bc	10.25±1.01 cd	8.49±0.96 ab	8.12±0.76 bc
	HN162	7.48±1.24 a	13.98±0.41 bc	9.68±0.65 cde	7.87±0.43 c	7.96±0.44 bc
	ZYS01	7.61±0.78 a	10.21±1.22 d	9.24±0.47 de	8.04±0.66 c	7.91±0.82 c

生理指标 Physical index	种质材料 Germplasm	对照 Control	处理Treatment
生理指标 Physical index	种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
超氧化物歧化酶活性 SOD activity/ （U·min^-1·g^-1 FW）	HLJ105	88.97±7.62 ab	179.46±12.35 a	142.69±8.24 ab	126.17±7.49 a	112.08±6.14 a
	RY126	94.66±8.54 a	192.54±14.28 a	148.12±9.83 a	121.54±6.24 ab	109.47±5.25 ab
	BY156	87.94±7.33 ab	183.67±16.91 a	145.84±7.56 a	119.88±8.33 ab	110.85±5.38 ab
	HLJ106	97.15±9.16 a	196.43±11.43 a	140.09±8.12 ab	126.46±6.42 a	106.97±6.43 ab
	RY102	89.41±8.27 ab	152.41±15.57 b	135.17±9.34 b	115.43±5.51 b	104.35±5.19 b
	BY141	86.52±8.19 b	146.59±13.66 b	122.54±8.55 c	118.42±6.85 ab	111.32±6.64 a
	RY114	96.33±9.24 a	142.17±11.08 b	119.53±6.71 c	117.43±5.97 ab	107.39±5.32 ab
	NM103	84.16±7.55 b	155.68±12.63 b	123.57±5.58 c	102.45±6.14 c	94.54±4.17 c
	XJ101	83.28±6.51 b	140.29±10.54 b	118.58±9.15 c	103.84±7.62 c	93.67±5.85 c
	JL154	86.66±5.89 b	152.15±14.56 b	120.64±8.11 c	100.21±4.68 cd	91.16±5.11 c
	LN181	85.29±9.83 b	148.96±16.87 b	116.81±5.49 c	98.45±7.39 cd	94.68±4.98 c
	SX100	84.82±7.04 b	153.91±12.16 b	117.35±6.97 c	95.54±8.11 cd	93.39±4.06 c

生理指标 Physical index	种质材料 Germplasm	对照 Control	处理Treatment
生理指标 Physical index	种质材料 Germplasm	对照 Control	3 ℃	5 ℃	7 ℃	9 ℃
超氧化物歧化酶活性 SOD activity/ （U·min^-1·g^-1 FW）	HLJ105	88.97±7.62 ab	179.46±12.35 a	142.69±8.24 ab	126.17±7.49 a	112.08±6.14 a
	RY126	94.66±8.54 a	192.54±14.28 a	148.12±9.83 a	121.54±6.24 ab	109.47±5.25 ab
	BY156	87.94±7.33 ab	183.67±16.91 a	145.84±7.56 a	119.88±8.33 ab	110.85±5.38 ab
	HLJ106	97.15±9.16 a	196.43±11.43 a	140.09±8.12 ab	126.46±6.42 a	106.97±6.43 ab
	RY102	89.41±8.27 ab	152.41±15.57 b	135.17±9.34 b	115.43±5.51 b	104.35±5.19 b
	BY141	86.52±8.19 b	146.59±13.66 b	122.54±8.55 c	118.42±6.85 ab	111.32±6.64 a
	RY114	96.33±9.24 a	142.17±11.08 b	119.53±6.71 c	117.43±5.97 ab	107.39±5.32 ab
	NM103	84.16±7.55 b	155.68±12.63 b	123.57±5.58 c	102.45±6.14 c	94.54±4.17 c
	XJ101	83.28±6.51 b	140.29±10.54 b	118.58±9.15 c	103.84±7.62 c	93.67±5.85 c
	JL154	86.66±5.89 b	152.15±14.56 b	120.64±8.11 c	100.21±4.68 cd	91.16±5.11 c
	LN181	85.29±9.83 b	148.96±16.87 b	116.81±5.49 c	98.45±7.39 cd	94.68±4.98 c
	SX100	84.82±7.04 b	153.91±12.16 b	117.35±6.97 c	95.54±8.11 cd	93.39±4.06 c