关键词: 基因打靶;正负筛选;codA;DT-A

Abstract:

Gene targeting is one of the important tools in reversed genetics and a powerful tool in functional characterization of plant genes. Positive-negative selection system is used to identify correct transformants upon gene targeting. Using selection systems can reduce work load and greatly improve gene targeting efficiency. In recent years, with continuous studies and improvement, the use of positive-negative selection in plant gene targeting has become normal. This paper expounds the principle of the method and summarized its research progress, which will be of significant value f or gene targeting study in the future.

Key words: gene targeting, positive-negative selection, codA, DT-A