关键词: 水稻;PA64S;细菌人工染色体;基因组文库

Abstract:

9311 and PA64S are the paternal and maternal cultivars of the super hybrid rice LYP9, respectively. The 9311 genome has been sequenced by Beijing Genomics Institute (BGI). However, for PA64S, no any public genomic resource is available to date. Research on PA64S genome will help in comparative studies between and cloning of agriculturally important genes from the two parental genomes 9311 and PA64S, facilitating the studies on LYP9 genome. In this paper, the first large insert and deep coverage BAC library for PA64S was constructed, taking the young tender leaf blade of PA64S as material. The library contains 37 632 clones with an average insert size of 138 kb, representing nearly 12-fold of rice genome. The empty-vector rate is only 1.5% and the mitochondrial and chloroplast DNA contamination rates are 0.35% and 1.85%, respectively. The clones have been deposited in 98 of 384-well plates and stored at -80℃ freezers, and are accessible to research communities as a public genome resource.

Key words: rice, PA64S, BAC, genomic DNA library