适于转基因产品检测的核酸扩增技术

doi:10.3969/j.issn.1008-0864.2011.06.13

›› 2011, Vol. 13 ›› Issue (6): 78-81.DOI: 10.3969/j.issn.1008-0864.2011.06.13

适于转基因产品检测的核酸扩增技术

刘信

(农业部科技发展中心, 北京 100122)

收稿日期:2011-11-01 修回日期:2011-11-23 出版日期:2011-12-15 发布日期:2011-12-15
作者简介:刘信,高级农艺师,研究方向为转基因生物安全评价与检测鉴定。Tel:010-59199385|E-mail:liuxin@agri.gov.cn
基金资助:
国家转基因生物新品种培育重大专项(2011ZX08012-003)资助。

Alternative DNA Amplification Methods Propitious to GMO Detection

LIU Xin

(Development Center for Science and Technology, Ministry of Agriculture, Beijing 100122, China)

Received:2011-11-01 Revised:2011-11-23 Online:2011-12-15 Published:2011-12-15

摘要/Abstract

摘要：

目前,转基因产品检测主要以核酸为对象,特别是外源重组DNA。靶DNA序列的扩增是转基因产品检测的核心技术,如已经广泛应用的定性、定量PCR技术。随着转基因作物种类的增多、种植范围的扩大,已有方法在某些情况下已不能完全满足检测要求,如高通量多靶标分析。综述了近年来基于核酸水平的检测新技术,并对其未来应用于转基因产品检测进行了分析。

关键词: 转基因生物检测;核酸扩增

Abstract:

Nucleic acids, especially forign recombinant DNA, are targets for analysis of genetically modified organisms (GMO). DNA amplification of targets has become the core technology in GMO detection, such as qualitative and quantitative PCR. Along with the increase of GMO and the expansion of planting range, original detection methods have some limitations such as lack of true multiplexing properties. Alternative nucleic acid amplification methods with promising characteristics are summarized in the paper. Special focus is given to the possibilities of using these alternative methods for GMO detection in future.

Key words: genetically modified organisms detection, DNA amplification

中图分类号:

TS207.3

刘信. 适于转基因产品检测的核酸扩增技术[J]. , 2011, 13(6): 78-81.

LIU Xin. Alternative DNA Amplification Methods Propitious to GMO Detection[J]. , 2011, 13(6): 78-81.

适于转基因产品检测的核酸扩增技术

Alternative DNA Amplification Methods Propitious to GMO Detection

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