关键词: 环介导等温扩增;依赖核酸序列的扩增;链替代扩增;滚环扩增

Abstract:

With the rapid development of biotechnology, a growing number of isothermal amplification technologies are applied in the field of molecular biology research. According to the dependent or independent on temperature cycling, nucleic acid amplification can be divided into two types: isothermal amplification and non-isothermal amplification. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), and rolling circle amplification (RCA), have gradually shown their potential for wider application, due to their advantages in high speed, sensitivity and independent on special instruments. This paper focuses on the principles, characteristics and applications of these isothermal amplification technologies, especially the applied possibility of these alternative methods on genetically modified organisms (GMOs)

Key words: LAMP, NASBA, SDA, RCA