关键词: 离子注入；多杀菌素；刺糖多孢菌；诱变选育

Abstract:

Spinosad are natural macrolide compound, produced by Saccharopolyspora spinosa with aerobic fermentation. It is a new bio-pesticide with expansive foreground. In this study, low energy nitrogen ion implantation technology and rational selection were used to obtain high-yield spinosad strain. The results indicated that the survival rate curve took a “saddle” shape and 30 s×2.6×1013 ions/cm2 implanted dose was used to further mutation and selection. According to the breeding of precursor and metabolite resistance, 5 high spinosad-producing mutants were achieved, the top of which was 44% higher than the initial strain. These showed that this technology is effective for high-yield fungus breeding.

Key words: ion implantation, spinosad, Saccharopolyspora spinosa, mutation breeding