›› 2012, Vol. 14 ›› Issue (4): 148-152.DOI: 10.3969/j.issn.1008-0864.2012.04.22.

• 方法与技术创新 • 上一篇    

利用离子注入技术选育多杀菌素高产菌株

郭伟群1,罗莉斯2,李能威1,张晓琳1   

  1. 1.国家粮食局科学研究院, 北京 100037; 
    2.贵州省油料研究所, 贵阳 550006
  • 收稿日期:2011-11-24 出版日期:2012-08-15 发布日期:2012-03-13
  • 通讯作者: 张晓琳,副研究员,博士,主要从事发酵生物技术研究。E-mail:zxl@chinagrain.org
  • 作者简介:郭伟群,助理研究员,硕士,主要从事发酵生物技术研究。E-mail:gwq@chinagrain.org。
  • 基金资助:

    国家粮食局科学研究院基本科研业务项目(ZX0906),“十二五”国家科技支撑计划项目(2011BAD03B02)资助。

Breeding High-yield Spinosad-producing Strain by Nitrogen Ion Implantation

GUO Wei-qun1, LUO Li-si2, LI Neng-wei1, ZHANG Xiao-lin1   

  1. 1.Academy of State Administration of Grain, Beijing 100037|
    2.Oil Crops Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
  • Received:2011-11-24 Online:2012-08-15 Published:2012-03-13

摘要:

多杀菌素是由刺糖多孢菌(Saccharopolyspora spinosa)发酵产生的大环内酯类化合物,是一种应用前景广阔的生物农药。采用氮离子注入方法并结合前体和自身代谢物抗性来选育多杀菌素高产菌株。结果显示利用氮离子注入诱变刺糖多孢菌,其致死率曲线呈典型的马鞍形,并确定30 s×2.6×1013 ions/cm2的注入剂量用于进一步诱变筛选。通过前体及自身代谢物抗性筛选,最终得到5株多杀菌素高产菌株,其多杀菌素的最高产量比选育前提高了44%。表明该技术对多杀菌素高产菌株的选育是一种行之有效的方法。

关键词: 离子注入;多杀菌素;刺糖多孢菌;诱变选育

Abstract:

Spinosad are natural macrolide compound, produced by Saccharopolyspora spinosa with aerobic fermentation. It is a new bio-pesticide with expansive foreground. In this study, low energy nitrogen ion implantation technology and rational selection were used to obtain high-yield spinosad strain. The results indicated that the survival rate curve took a “saddle” shape and 30 s×2.6×1013 ions/cm2 implanted dose was used to further mutation and selection. According to the breeding of precursor and metabolite resistance, 5 high spinosad-producing mutants were achieved, the top of which was 44% higher than the initial strain. These showed that this technology is effective for high-yield fungus breeding.

Key words: ion implantation, spinosad, Saccharopolyspora spinosa, mutation breeding

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