紫茎泽兰热激蛋白70基因的克隆与序列分析

doi:10.3969/j.issn.10080864.2010.

›› 2010, Vol. 12 ›› Issue (1): 111-117.DOI: 10.3969/j.issn.10080864.2010.

紫茎泽兰热激蛋白70基因的克隆与序列分析

肖艳萍1,2,3,宫伟娜2,万方浩2,李正跃1,谢丙炎3

(1云南农业大学植物保护学院, 昆明 650201|2.中国农业科学院植物保护研究所, 北京 100094|3.中国农业科学院蔬菜花卉研究所,北京 100081)

收稿日期:2009-10-16 修回日期:2010-01-03 出版日期:2010-02-15 发布日期:2010-01-28
通讯作者: 万方浩,研究员,博士,主要从事入侵生物学研究。Email:wanfh@caas.net.cn
作者简介:肖艳萍,硕士研究生,研究方向为农业昆虫与害虫防治。Email: yanping_xiao@163.com
基金资助:
国家973计划项目(2009CBI19200)资助。

Cloning and Sequence Analysis of Heat Shock Protein 70 Gene from Ageratina adenophora(Eupatorium adenophorum)

XIAO Yan-ping1,2,3, GONG Wei-na2, WAN Fang-hao2, LI Zheng-yue1, XIE Bing-yan3

(1.College of Plant Protection, Yunnan Agricultural University, Kunming 650201|2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081|3.Institute of Vegetables and Flowers, CAAS, Beijing 100081, China)

Received:2009-10-16 Revised:2010-01-03 Online:2010-02-15 Published:2010-01-28

摘要/Abstract

摘要：

热激蛋白70(HSP70)与植物的抗逆性密切相关。根据其他植物hsp70的保守性区域设计特异性引物，采用RTPCR方法从紫茎泽兰Ageratina adenophora中克隆到hsp70基因全长cDNA序列。序列分析结果表明，该cDNA包含1个 1 938 bp的开放阅读框(GenBank登录号:FJ598132),共编码645个氨基酸，相对分子质量为70.58 kDa，命名为hsp70.58。该蛋白包含真核生物HSP70高度保守的三个家族标签和末尾基序EEVD。运用实时定量PCR 分析，探测到hsp70.58在mRNA水平上皆能被高温和低温诱导表达，说明该基因属于诱导型hsp70基因。

关键词: 紫茎泽兰;热激蛋白;hsp70;克隆

Abstract:

Heat shock protein 70 (HSP70) plays an essential role in plant stress resistance. According to the conserved regions of hsp70 from other plant species, a pair of specific primers were designed. A fulllength cDNA of hsp70 gene from Ageratina adenophora was cloned by the technique of RTPCR. Its ORF (open reading frame) (GenBank locus: FJ598132) named hsp70.58 was 1 938 bp. It encoded a polypeptide of 645 amino acids with an estimated molecular mass of 70.58 kDa and contained the three highly conserved HSP70 family signatures and Cterminal pentapeptide EEVD. Relative realtime RTPCR was used to analyze the expression of hsp70.58 gene after heat shock and cold shock, respectively. The results showed that mRNA expression of hsp70.58 was significantly upregulated after temperature stress. It indicated that this gene belonged to hsp70 inducible type.

Key words: Ageratina adenophora (Syn. Eupatorium adenophorum), Heat shock protein, hsp70, clone

中图分类号:

肖艳萍1,2,3,宫伟娜2,万方浩2,李正跃1,谢丙炎3. 紫茎泽兰热激蛋白70基因的克隆与序列分析[J]. , 2010, 12(1): 111-117.

XIAO Yan-ping1,2,3, GONG Wei-na2, WAN Fang-hao2, LI Zheng-yue1, XIE Bing-yan3. Cloning and Sequence Analysis of Heat Shock Protein 70 Gene from Ageratina adenophora(Eupatorium adenophorum)[J]. , 2010, 12(1): 111-117.

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紫茎泽兰热激蛋白70基因的克隆与序列分析

Cloning and Sequence Analysis of Heat Shock Protein 70 Gene from Ageratina adenophora(Eupatorium adenophorum)

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