OsCtBP-A基因过量表达和RNAi沉默对水稻根系生长和抗性的影响

doi:10.3969/j.issn.1008-0864.2010.

›› 2010, Vol. 12 ›› Issue (1): 106-110.DOI: 10.3969/j.issn.1008-0864.2010.

OsCtBP-A基因过量表达和RNAi沉默对水稻根系生长和抗性的影响

吴茂森,李磊,吴静,陈华民,何晨阳

(中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193)

收稿日期:2009-11-29 修回日期:2009-12-14 出版日期:2010-02-15 发布日期:2010-01-26
通讯作者: 何晨阳,研究员,博士,研究方向为分子植物病理学。Tel:010-62894147;E-mail:cyhe@caas.net.cn
作者简介:吴茂森,副研究员,博士,从事水稻抗/感病功能基因的研究。E-mail:mswu@ippcaas.cn
基金资助:
国家转基因生物新品种培育重大专项(2009ZX08009-044B,2008ZX08001-002);国家重点实验室自主研究课题专项(SKL2009SR03)资助。

Effects of Overexpression and RNAi-mediated Silence of OsCtBP-A Gene on Root Growth and Stress Resistance in Rice

WU Mao-sen, LI Lei, WU Jing, CHEN Hua-min, HE Chen-yang

(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Received:2009-11-29 Revised:2009-12-14 Online:2010-02-15 Published:2010-01-26

摘要/Abstract

摘要：

为了阐明己克隆的水稻C端结合蛋白家族基因OsCtBP-A的功能,构建了该基因的过量表达和RNAi沉默载体,通过农杆菌介导转化水稻品种日本晴愈伤组织,分别获得了45个过量表达和75个RNAi 沉默T0代转基因植株,并进行了PCR和GUS染色鉴定。T1代过量表达株OsCtBP-A基因表达量明显增加,苗期根系生长增加,对水稻白叶枯病抗性无明显变化；而基因沉默株OsCtBP-A基因表达量显著下降,苗期根系发育迟缓,抗旱性明显降低,抗病性减弱。表明OsCtBP-A基因的表达可能对水稻苗期根系发育、抗旱性和抗病性具有不同程度的调控作用。

关键词: OsCtBP-A;过量表达;RNAi沉默;根系发育;抗性

Abstract:

In order to expound the functions of cloned C-terminal binding protein gene OsCtBP-A, we established its overexpression and RNAi constructs and transformed them into calli of rice (cv. Nipponbare) via Agrobacterium-mediated transformation. Forty-five transgenic plants with overexpression and 75 with RNAi were generated and identified by PCR and GUS staining analysis. In T1 progeny of transgenic rice lines with overexpression, OsCtBP-A gene expression was remarkably increased, and root development was enhanced during seedling stage, while its resistance to rice leaf blight was no much changed. In contrast, OsCtBP-A gene expression was significantly reduced, and the root development at seedling stage was slowed down and its resistance to drought was weakened in RNAi silenced lines. The results suggested that OsCtBP-A gene might be involved in regulation of root development at seedling stage and resistances to drought and diseases.

Key words: OsCtBP-A, overexpression, RNAi-mediated silence, root development, stress resistance

中图分类号:

Q786

吴茂森,李磊,吴静,陈华民,何晨阳. OsCtBP-A基因过量表达和RNAi沉默对水稻根系生长和抗性的影响[J]. , 2010, 12(1): 106-110.

WU Mao-sen, LI Lei, WU Jing, CHEN Hua-min, HE Chen-yang. Effects of Overexpression and RNAi-mediated Silence of OsCtBP-A Gene on Root Growth and Stress Resistance in Rice[J]. , 2010, 12(1): 106-110.

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OsCtBP-A基因过量表达和RNAi沉默对水稻根系生长和抗性的影响

Effects of Overexpression and RNAi-mediated Silence of OsCtBP-A Gene on Root Growth and Stress Resistance in Rice

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