关键词: 外源基因;基因表达效率;转基因植物

Abstract:

In transgenic research and production practice, researchers often expect target gene can efficiently express in transgenic organisms, so as to achieve the objective-obtaining crops highly resistant to insecticide and herbicide, or using plants as bioreactor to produce vaccines, antibiotics and other medical products and industrial proteins, including enzymes with high added-value etc. In this paper, by a lot of research literatures, we reviewed various strategies, which could enhance the efficiency of foreign gene expression. From the integration of the foreign gene, the regulation of foreign gene in transcription level and the protein separation after the foreign gene expression selecting 3 levels to illustrate the advantages and disadvantages of relevant strategies, so as to offer reference for the construction of expressive vectors in transgenic research.

Key words: foreign gene, gene expression efficiency, transgenic plant