›› 2010, Vol. 12 ›› Issue (4): 95-100.DOI: 10.3969/j.issn.1008-0864.2010.04.18

• 研究报告 • 上一篇    下一篇

水稻L-半乳糖途径相关基因的表达特性

邓载安1,2,3,张治礼1,2,4,张执金3,黄荣峰3   

  1. (1.海南大学, 海南 儋州 571001|2.中国热带农业科学院热带生物技术研究所, 海口 571101|3.中国农业科学院生物技术研究所, 北京 100081|4.海南省农业科学院, 海口 571000)
  • 收稿日期:2010-04-16 修回日期:2010-06-15 出版日期:2010-08-15 发布日期:2010-07-14
  • 通讯作者: 黄荣峰,主要从事植物分子生物学与基因工程研究。Tel:010-82106143;E-mail:rfhuang@caas.net.cn
  • 作者简介:邓载安,硕士研究生,主要从事植物生物学研究。
  • 基金资助:

    国家973计划项目(2007CB108801)资助。

Gene Expression Characteristics Related to L-Galactose Pathway in Rice

DENG Zai-an1,2,3, ZHANG Zhi-li1,2,4, ZHANG Zhi-jin4, HUANG Rong-feng3   

  1. (1.Hainan University, Hainan Danzhou 571001|2.Institute of Tropical Bioscience and Biotechnology, Haikou 571101| 3.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081|4.Hainan Academy of Agricultural Sciences, Haikou 571000, China)
  • Received:2010-04-16 Revised:2010-06-15 Online:2010-08-15 Published:2010-07-14

摘要:

维生素C又称抗坏血酸(AsA),在植物中不仅是重要的抗氧化剂,还具有许多其它重要的生理功能。研究发现L-半乳糖途径是双子叶植物拟南芥AsA合成的主要途径,但单子叶植物水稻中AsA合成的L-半乳糖途径的功能和调控机理还不清楚。通过同源序列比对,在水稻基因组中检索到与拟南芥L-半乳糖途径相关酶的同源基因,通过分析水稻根与叶中及持续光、暗处理下叶片中AsA含量变化及L-半乳糖途径中相关酶基因的表达,初步探究了水稻L-半乳糖途径中调控AsA合成的关键酶基因。

关键词: 水稻(Oryza sativa);抗坏血酸;L-半乳糖途径;基因表达分析

Abstract:

Vitamin C or ascorbic acid (AsA), is not only an important antioxidant, but also an active substance with many other physiological functions. The L-galactose pathway has been proven to be the key pathway of ascorbic acid in Arabidopsis thaliana. However, limited information about L-galactose pathway of ascorbic acid synthesis in rice is reported. In this research, through sequence alignments, the homologous genes putatively encoding rice L-galactose pathway enzymes were obtained. Then based on analysis of tissue specificity and leaf treatment with continuous light or darkness, AsA content and gene expression related to L-galactose pathway enzyme were determined, preliminarily evidencing the key genes controlling the synthesis of AsA in rice.

Key words: rice(Oryza sativa), ascorbic acid, L-galactose pathway, gene expression

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