关键词: TLR4基因 PCR-RFLP 体细胞评分 乳房炎

Abstract:

To detect the DNA variation of bovine TLR4 gene, a 321bp segment of TLR4 gene exon m was analyzed by PCR-RFLP. The resuhs showed that a mutation exists in the amplified product, which can be recognized by restriction endonuclease Bbv12 1 and induced that changed Thr into Ile. Both allele C and T were found in the population and this was a moderate polymovphism, but C was dominant allele. In addition, the correlation of the genotype and dailry mastitis was also analyzed in this experiment by the least square analysis. The resuhs showed that the SCS of different genotypes had the trend of CC 〉 CT 〉TT. But statistical results indicated that SCS was not significantly affected by different genotypes （ P 〉 0.05 ）.

Key words: TLR4 gene , PCR-RFLP , SCS , mastitis