新疆天山雪莲根部冻土产酶微生物的初步研究

›› 2008, Vol. 10 ›› Issue (2): 82-87.

新疆天山雪莲根部冻土产酶微生物的初步研究

张国华[1,2] 罗会颖[2] 陈劲春[1] 姚斌[2]

[1]北京化工大学生命科学与技术学院,北京100029 [2]中国农业科学院饲料研究所,北京100081

收稿日期:2007-12-27 修回日期:2008-01-21 出版日期:2008-04-15 发布日期:2009-07-29
通讯作者: 姚斌，研究员，博士，博士生导师，从事微生物工程研究。Tel：010-68975126；E-mail：yaobin@mail．caas．net．cn。
作者简介:张国华|硕士研究生|从事微生物工程研究。陈劲春|教授|博士|博士生导师|从事生物制药研究。
基金资助:
国家948项目（2007-Z3）资助.

Pilot Studies on Enzyme-Producing Microflora in Frozen Soil at the |Root of Saussureae involucratae Kar. et Kir. et Maxim from Tianshan, Xinjiang Autonomous Region

ZHANG Guo-hua, LU0 Hui-ying, CHEN Jin-chun| YA0 Bin

1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029; |2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081 China

Received:2007-12-27 Revised:2008-01-21 Online:2008-04-15 Published:2009-07-29

摘要/Abstract

摘要：

利用不同培养基对新疆天山雪莲根部冻土微生物进行了分离与培养，通过16S或18SrDNA序列扩增初步鉴定了分离所得微生物，并构建系统发育树。结合选择性培养基进行了产蛋白酶、产甘露聚糖酶、产木聚糖酶、产纤维素酶和产淀粉酶等微生物的筛选。结果表明，新疆天山雪莲根部冻土中蕴含着丰富的产酶微生物，分离得到的34株细菌中大部分都能产酶，其中以产淀粉酶为主，部分产纤维素酶、甘露聚糖酶和木聚糖酶。产淀粉酶细菌主要属于Pseudeomonas，Flavobacterium和Arthrobacter等属，产纤维素酶细菌主要属于Pseudeomonas和Arthrobacter等属。分离得到的5株真菌，以产淀粉酶和木聚糖酶为主。

关键词: 雪莲根部冻土产酶微生物

Abstract:

The microflora in the frozen soil at the root of Saussureae Involucratae Kar. et Kir. et Maxim from Tianshan in Xinjiang Autonomous Region were isolated under different culture conditions and identified by 16S or 18S rDNA sequences amplification, the neighbor-joining phylogenetic tree was then constructed. The microflora producing all kinds of enzymes including proteinase, mannanasc, xylanase, cellulase and amylase etc, were screened by selective media. The results showed that rich enzyme-producing microflora existed in the frozen soil. Among 34 bacteria strains isolated, most of them mainly excreted amylase, and partly excreted cellulose degrading enzyme, such as mannanase, protein degrading enzyme and xylanase. The strains which excreted amylase are mainly in genus of Pseudeomonas, Flavobacteriu and Arthrobacter, while the bacteria that excreted cellulase belong to Pseudeomonas and Arthrobacter. Five fungi strains isolated from the frozen soil can produce amylase or xylanase.

Key words: Saussureae involucratae Kar. et Kir. et Maxim , frozen soil near root, enzyme-producing microflora

中图分类号:

Q93-3 Q949.783.5

张国华,罗会颖,陈劲春,姚斌. 新疆天山雪莲根部冻土产酶微生物的初步研究[J]. , 2008, 10(2): 82-87.

ZHANG Guo-hua, LU0 Hui-ying, CHEN Jin-chun| YA0 Bin. Pilot Studies on Enzyme-Producing Microflora in Frozen Soil at the |Root of Saussureae involucratae Kar. et Kir. et Maxim from Tianshan, Xinjiang Autonomous Region[J]. , 2008, 10(2): 82-87.