关键词: 花培;同源四倍体;粳稻;诱导率

Abstract:

ice (Oryza sativa L.) anther culture was usually focused on diploid materials, thus it is meaningful to study the efficiency

of anther culture of tetraploid rice. Two japonica autotetraploid rice varieties, linglun and 02428, and their corresponding

diploids were used in the present work. A total of 22 induction medium was designed and divided into 3 groups to test the

effects of variety and concentration of sugar, concentration of agar, variety of basal media and hormone. The results

indicated that the combination of 6% sucrose and 0.75% agar was the best one for the callus inductivity of autotetraploid

rice,  N6 and NB with sucrose are more suitable for the anther callus induction in tetraploid rice.  It was found that the

improvement of inductivity in tetraploid rice was 2-fold than that of diploid rice by adding the hormones with the

concentration of 0.5 mg/L.However, kinetics was proved to be better for linglun and isopentenyladenine is better for 02428.

On the whole, significant difference of induction rate exists between tetraploid and diploid materials under the same culture

conditions. However, no coherent pattern was found when compared different varieties for the same medium, which indicates

that genotype is always a key factor in anther culture of rice. In conclusion, more factors should be considered for

enhancing the inductivity of certain rice varieties.

Key words: anther culture, autotetraploid, japonica rice, inductivity