关键词: 水稻 Whirly转录因子 RNA干扰 过敏反应（HR） 负调控因子

Abstract:

The regulation function of rice Whirly transcription factor for rice hypersensitive response （HR） induced by non-host pathogen bacterium was studied with RNAi technology. A fragment of Oswhirly gene encoding rice Whirly transcription factor was amplified by PCR method, then inserted into 2 flanking MCSs of pTCK303 vector in forward or reverse direction. The constructed vector pTCK-Oswhy was the RNAi vector for Oswhirly gene silence and was used to transform rice cells mediated by the Agrobacterium tumefaciens EHA105 strain. Results showed that hypersensitive response was obviously induced for pTCK303-Oswhy transformed rice cells after inoculation with non-host pathogen bacterium. The cell death rate of transformed rice cells was significantly higher than that of wild type or empty vector transformed rice cells. These results suggested that the Whirly transcription factor might be a negative regulator for the control of the rice induced hypersensitive response.

Key words: rice, Whirly transcription factor, RNA interference, hypersensitive response（HR） ;negative regulator