关键词: 大豆蛋白 ACE抑制肽 高效液相色谱 酶解 水解度

Abstract:

Four types of enzymes were used to hydrolyze prepared soybean protein isolates and to study degree of hydrolysis and ACE （ angiotensin I-converting enzyme） inhibitory activity of the product. The results showed that alcalase was the optimum enzyme to produce soybean peptide. The optimum alcalase hydrolysis process for producing ACE inhibitory peptides was under 60 ℃ ,with pH 8.0, 4% substrate concentration,4% enzyme concentration, and 14.4% hydrolysis degree. After optimization, the ACE inhibitory activity can reach 84.1%.

Key words: soybean protein, angiotensin I converting enzyme inhibitory peptide, high performance liquid chromatograpy（HPLC） , enzymatic hydrolysis , degree of hydrolysis