关键词: 高分子量DNA 细菌人工染色体 箝位匀强电场电泳

Abstract:

In order to construct bacterial artificial chromosomes library of Streptomyces albulus var.wuyiensis,we extractd high molecular weight DNA(HMW-DNA) from Streptomyces albulus var.wuyiensis.Mycelium pellet of Streptomyces albulus var.wuyiensis was embeded in low melting point agarose.The agarose block was treated by lysozyme,proteinase K and restriction enzyme.Contour-clamped homogeneous electric field(CHEF) gel electrophoresis was performed for prepared DNA plugs.Electrophoresis analysis shows no degradation...

Key words: high molecular weight DNA, bacterial artificial chromosomes library, CHEF gel electrophoresis;