费氏丙酸杆菌谢氏亚种和植物乳杆菌中亚油酸异构酶的分离纯化

›› 2009, Vol. 11 ›› Issue (2): 49-55.

费氏丙酸杆菌谢氏亚种和植物乳杆菌中亚油酸异构酶的分离纯化

王丽敏^1,2,吕加平¹,刘鹭¹

1.中国农业科学院农产品加工研究所, 农业部农产品加工与质量控制重点开放实验室, 北京 100193|2.中国科学院微生物研究所, 北京 100190

收稿日期:2008-09-27 修回日期:2009-03-06 出版日期:2009-04-15 发布日期:2009-04-15
通讯作者: 吕加平,研究员,主要从事乳品加工及微生物方面的研究。Tel：010-62815542; E-mail：lvjp586@vip.sina.com
作者简介:王丽敏,助理研究员,博士,主要从事工业微生物学研究。
基金资助:
国家863计划项目（2006AA10Z345和2006AA10Z302 ）资助。

Isolation and Purification of Linoleate Isomerase from P. freudenreichii ssp. shermanii and L. plantarum

WANG Li-ming^1,2, LV Jia-ping¹, LIU Lu¹

1. Key Laboratory of Agro-food Processing and Quality Control, MOA, Institnte of Agro-food
Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193; 2.Institute of Microbiology, Chinese Academy of Science, Beijing 100190, China

Received:2008-09-27 Revised:2009-03-06 Online:2009-04-15 Published:2009-04-15

摘要/Abstract

摘要：

采用盐析、Sephacryl S-200HR凝胶过滤层析、DEAE Sepharose F.F.离子交换层析相结合的方法，分别建立了来源于费氏丙酸杆菌谢氏亚种（P. feudenreichii ssp. shermanii）和植物乳杆菌（L. plantarum）的亚油酸异构酶的分离提取步骤。结果表明不同来源的亚油酸异构酶在纯化的过程中表现出不同的洗脱特性，P. freudenreichii ssp. shermanii亚油酸异构酶的分子质量约为56 kDa, L. plantarum亚油酸异构酶的分子质量为50.65 kDa。不同来源亚油酸异构酶的分离纯化方法存在差异。

关键词: 共轭亚油酸（CLA）；费氏丙酸杆菌费氏亚种（P. freudenreichii ssp. shermanii）；植物乳杆菌（L. plantarum）；亚油酸异构酶

Abstract:

Using saltingout method, combined with Sephacryl S-200HR gel filtration chromatography and DEAE Sepharose F.F. ion exchange chromatography, linoleate isomerase was isolated and purified from P. freudenreichii ssp. shermanii and L. plantarum, respectively. linoleate isomerase from different sources showed different elution characteristics in the purification process. The molecular weight of linoleate isomerase from P. freudenreichii ssp. shermanii was 56 kDa, and that from L. plantarum was 50.65 kDa. The result indicated that big differences existed in the purification method of linoleate isomerase due to their different sources.

Key words: conjugated linoleic acid (CLA);P. freudenreichii ssp. shermanii; , L. plantarum, linoleate isomerase

中图分类号:

Q939.97

王丽敏,吕加平,刘鹭. 费氏丙酸杆菌谢氏亚种和植物乳杆菌中亚油酸异构酶的分离纯化[J]. , 2009, 11(2): 49-55.

WANG Li-ming, LV Jia-ping, LIU Lu. Isolation and Purification of Linoleate Isomerase from P. freudenreichii ssp. shermanii and L. plantarum[J]. , 2009, 11(2): 49-55.