关键词: 灰霉病菌；枯草芽孢杆菌；鉴定；培养基优化；生防细菌BAB-1

Abstract:

Antigonistic bacterium BAB-1 was isolated from soil and showed potential for controlling tomato grey mold. Strain BAB-1 was identified as Bacillus subtilis according to its morphological characteristics, 16S rDNA sequence and the result of API 50CHB system. 16 different media for the bacterial growth and production of antifungal substrates were tested. Results showed that the medium No.3 was suitable for either bacterial growth or antifungal substrates producing. Optimum fermentation conditions for bacterial growth were as following: temperature 30℃, 210 rpm, pH 7.24, inoculation amount 3% and amount of loading 70 mL/250 mL flask. Under these conditions, the cell density could reach 1.63×109 cfu/mL. While the optimum conditions for antifungal substance producing were temperature 30℃, 210 rpm, pH 7.24, inoculation amount 2% and amount of loading 100 mL/250 mL flask. Under these conditions, the inhibition zone of strain BAB-1 against Botrytis cinerea could reach 1.81cm width.

Key words: Botrytis cinerea, Bacillus subtilis, ideutification, media optimization, biocontrol bacterial strain BAB-1