关键词: 产蛋下降致病病原;基因芯片;鉴别诊断;临床应用

Abstract:

The conserved sequences of new castle disease virus, avian influenza, infectious bronchitis virus, egg drop syndrome, infectious laryngotracheitis and mycoplasma gallisepticum were obtained by polymerase chain reaction （PCR） or reverse transcription-polymerase chain reaction (RT-PCR). A two-dimensional DNA microarray, that is gene chip, was produced by using cellulose membrane as  bio-chip substrate and micro-spotting technology. Pathogen polynucleotide was extracted from suspected samples, then labeled with bio-11-dUTP by RT-PCR incorporation method. The labeled PCR product and prepared gene chip were hybridized. The rapid and efficient detection and analysis were achieved by detecting the strength of hybridization signals.  The gene chip sensitivity is higher than that of RT-PCR and virus isolation, by 102 and 104 fold, respectively. It has faithful specificity. The coincidence rate of animal experiments was over 95%.

Key words: egg drop syndrome pathogen, gene chip, identification diagnosis, clinical application