盐度胁迫对刺参非特异性免疫酶的影响

doi:10.3969/j.issn.1008-0864.2013.03.23

›› 2013, Vol. 15 ›› Issue (3): 163-168.DOI: 10.3969/j.issn.1008-0864.2013.03.23

盐度胁迫对刺参非特异性免疫酶的影响

王冲1,田燚2*,常亚青2,陈百尧3,4,伏光辉3,4,马衍平2

(1.瓦房店市海洋与渔业局, 辽宁瓦房店116300|2.大连海洋大学, 农业部北方海水增养殖重点开放实验室, 辽宁大连 116023|3.连云港市海洋与水产科学研究所, 江苏连云港 222044|4.连云港市海珍品增养殖试验场, 江苏连云港 222044)

收稿日期:2012-10-16 修回日期:2013-01-10 出版日期:2013-06-15 发布日期:2013-06-15
通讯作者: 田燚, 副教授,主要从事水产养殖研究。E-mail: tianyi@dlou.edu.cn
作者简介:王冲,高级实验师,主要从事水产养殖研究。E-mail: 13384110991@163.com。
基金资助:
国家863计划项目(2012AA10A412);国家自然科学基金项目（41106128）;江苏省科技支撑计划项目(BE2012421)资助。

Effect of Salinity Stress on Immune Enzyme Activity of Sea Cucumber (Apostichopus japonicus)

WANG Chong1, TIAN Yi2*, CHANG Ya-qing2, CHEN Bai-yao3,4, FU Guang-hui3,4, MA Yan-ping2

(1.Ocean and Fishery Bureau of Wafangdian City, Liaoning Wafangdian 116300|2. School of Life Science and Technology, Dalian Fisheries University, Liaoning Dalian 116023|3.Ocean and Fishery Science Research Institute of Lianyungang City, Jiangsu Lianyungang 222044|4.Seafood Breeding Farm of Lianyungang City, Jiangsu Lianyungang 222044, China)

Received:2012-10-16 Revised:2013-01-10 Online:2013-06-15 Published:2013-06-15

摘要/Abstract

摘要：

盐胁迫后刺参的行为表现及免疫酶活性会发生相应变化,以达到适应盐度变化的目的。设定5个盐度梯度(18、23、33、36和40),分析盐度胁迫后刺参行为变化及不同响应时间下体腔液中碱性磷酸酶（AKP）、酸性磷酸酶（ACP）、溶菌酶（LSZ）、超氧化物歧化酶（SOD）的活力变化。实验结果发现盐度增加和降低都使刺参的活动受到影响,低盐度胁迫比高盐度胁迫对刺参的影响大。碱性磷酸酶和酸性磷酸酶活性总体呈现先降低后升高,随着时间的延长逐渐恢复适应的趋势。盐度23溶菌酶活力显著高于盐度32的对照组;盐度为36时,溶菌酶活力在第1天最高,随后降低,维持2 d后,酶活力又开始升高,并高于盐度32的对照组水平;盐度为18、40时,酶活力显著低于对照组,并一直维持在较低水平。低盐18、23胁迫时,超氧化物歧化酶SOD酶活力明显低于对照组,且最低点均出现在第8 d。高盐36、40胁迫时,酶活力明显高于对照组,最高点分别出现在第5 d和第8 d。研究结果为刺参机体在盐度胁迫下的调节适应机制研究工作奠定一定的基础。

关键词: 刺参;盐度胁迫;酸性磷酸酶;碱性磷酸酶;SOD酶;溶菌酶

Abstract:

The behavior of sea cummber and the activities of enzyme would change under salt stress to adapt the environment. The sea cucumbers were temporarily cultured at different salinity levels 18, 23, 33, 36 and 40. The enzyme activity of alkaline phosphatase (AKP), acid phosphatase (ACP), lysozyme (LSZ) and superoxide dismutase (SOD) of sea cucumber were detected under different salinity stress. Survival was examined after the larvae were exposed to the salinity levels. The results indicated that salinity stress could affect sea cucumber activity and the lower salinity had more effect than the higher one. The activity of acid phosphatase (ACP) and alkaline phosphatase (AKP) increased firstly then decreased. The activity of SOD at 18 and 23 were significantly lower than the control group. The activities of superoxide dismutase at 36 and 40 were significantly higher than that in the control group. The results would provide certain foundation on salinity regulation mechanism of sea cucumber.

Key words: sea cucumber, salinity stress, scid phosphatase, alkaline phosphatase, lysozyme, superoxide dismutase

中图分类号:

S968.9

王冲1,田燚2*,常亚青2,陈百尧3,4,伏光辉3,4,马衍平2. 盐度胁迫对刺参非特异性免疫酶的影响[J]. , 2013, 15(3): 163-168.

WANG Chong1, TIAN Yi2*, CHANG Ya-qing2, CHEN Bai-yao3,4, . Effect of Salinity Stress on Immune Enzyme Activity of Sea Cucumber (Apostichopus japonicus)[J]. , 2013, 15(3): 163-168.

盐度胁迫对刺参非特异性免疫酶的影响

Effect of Salinity Stress on Immune Enzyme Activity of Sea Cucumber (Apostichopus japonicus)

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